TABLE 3.

Internalization efficiencies of C. freundii strain 3009 and recombinant E. coli HB101 strains expressing different adhesin determinants with human bladder epithelial cell lines T24 and RT112

Plasmid or strainEncoded adhesinaInternalization efficiencyb with:
T24 cellsRT112 cells
pBR322None0.0200.0007
pPIL110-54F1C0.020<0.0007
pMMP658-6Sfr0.007<0.0007
pANN801-13SfaI0.010<0.0007
pAZZ50SfaII0.0500.0008
pGB30FimEc0.020<0.001
pISF101FimSt0.002NDc
pTO3FimCf9.73.1
C. freundii 3009FimCf11.427.3
  • a FimEc, type 1 pili from E. coli; FimSt, type 1 pili from S. enterica serovar Typhimurium; FimCf, type 1 pili from C. freundii.

  • b The internalization efficiency is given as the percentage of the inoculum surviving gentamicin treatment. Data are the results of one of three indepently performed assays with similar outcomes.

  • c ND, not determined.