TABLE 2.

In vitro invasion efficiencies for different cell lines by C. freundii, S. enterica serovar Typhimurium, and recombinant E. coli carrying various plasmids or vector controls

Cell lineHuman origin% Invasiona
C. freundiiS. enterica serovar Typhimurium C17E. coli HB101 carrying:
30093009-dzpTO3pPH1pB7-3pSU19pBR322
T24Bladder11.4 ± 6.62.2 ± 1.952.1 ± 23.09.7 ± 6.86.1 ± 2.25.3 ± 2.60.05 ± 0.040.01 ± 0.01
RT112Bladder27.3 ± 4.12.1 ± 0.8NDb9.9 ± 2.1NDND0.02 ± 0.010.03 ± 0.02
HCT8Ileocecum67.2 ± 29.6ND41.4 ± 39.33.7 ± 1.71.0 ± 0.63.3 ± 1.40.05 ± 0.040.01 ± 0.03
HBMECcBrain3.3 ± 1.2NDNDND2.6 ± 1.3NDND0.01 ± 0.01
  • a Data are the means ± standard deviations from at least three independently performed invasion assays.

  • b ND, not determined.

  • c HBMEC, human brain microvascular endothelial cells.