TABLE 2.

Oligonucleotide sequences used for the study of binding activities of PlcR proteins

OligonucleotideSequencea
Upper strand
    T15′-TTATATATATTATGCATTATTTCATATCAAAAATTG-3′
    T25′-GTTATAATGATATTAAAATCTGCATATTTTAATTTA-3′
    T35′-GTTATAATGATATTAAAATCCGCATATTTTAATTTA-3′
    NS5′-CTCTGGTCTACCGCACCCGATTCTACTCTGC-3′
Lower strand
    T13′-AATATATATAATACGTAATAAAGTATAGTTTTTAAC-5′
    T23′-CAATATTACTATAATTTTAGACGTATAAAATTAAAT-5′
    T33′-CAATATTACTATAATTTTAGGCGTATAAAATTAAAT-5′
    NS3′-GAGACCAGATGGCGTGGGCTAAGATGAGACG-5′
  • a Sequences related to the PlcR consensus 5′-TATG.A....T.CATA-3′ are indicated in boldface. Nucleotides within this consensus sequence that differ from T1 are shown in italic.