TABLE 1.

Plasmids and strains used in this study

Plasmid or strainRelevant characteristic(s)aSource or reference
Plasmids
    pUTE29-plcR-papRB. cereus 569 plcR-papR DNA fragment inserted into pUTE2916
    pUTE29-plcRpUTE29-plcR-papR without papR geneThis work
    pUTE29-papRpUTE29-plcR-papR without plcR geneThis work
    pFP12pUTE29-plcR fusion to papRThis work
    pAE5Encodes B. cereus 569 plcR under control of B. anthracis protective antigen gene promoter16
Strains
    B. anthracis
        Sterne 34F2 DeltaT (=SdT)Sterne 34F2 cured of pXO1; therefore, pXO1pXO26
        Ames 33pXO1 pXO2 Ames derivative strain16
        SdT1SdT electroporated with pUTE29-plcR-papR; Tcr; does not produce intracellular PlcR; nonhemolytic strain16
        SdT2SdT(pAE5); Kmr; produces intracellular B. cereus PlcR; weakly hemolytic16
        SdT5SdT electroporated with pUTE29-plcR; Tcr; does not produce intracellular PlcR; nonhemolyticThis work
        SdT6SdT electroporated with pUTE29-papR; Tcr; does not produce intracellular PlcR; nonhemolyticThis work
        SdT12SdT electroporated with pFP12; Tcr; produces intracellular PlcR-PapR fused protein; hemolyticThis work
        A12Ames 33 electroporated with pFP12; Tcr; produces intracellular PlcR-PapR fused protein; hemolyticThis work
    E. coli
        XL2-BluerecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lac1qZΔM15 Tn10 (Tcr) Amy Cmr]Stratagene
        SCS110rpsL (Smr) thr leu endA thi-1 lacY galK galT ara tonA tsx dam dcm supE44D (lac-proAB) [F′ traD36 proAB lac1qZΔMIS]Stratagene
  • a Kmr, kanamycin resistant; Tcr, tetracycline resistant.