TABLE 1.

Primers, probes, annealing temperatures, and number of cycles used for RT-PCR analysis for genes of interest

GeneOligonucleotidesaProduct size (bp)Annealing temp (°C)No. of cycles
Reg3αFwd, 5′-TGGGAGTGGAGTAACTCCGAT; Rev, 5′-AAAGCAAGTACAGCCTTGCCG; Probe, 5′-GGGAGACTATTACTGCGATGGGA2575528
Reg3βFwd, 5′-ATCTACTGCCTTAGACCGTGCTT; Rev, 5′-AGAATTGAGCCCAAACTTATACC; Probe, 5′-AGACAGCAAACATCCCGAATT2946032
Reg3γFwd, 5′-TTACATCAACTGGGAGACGAATC; Rev, 5′-TGAGAATTAAGGCCATAGTGCAC; Probe, 5′-AACATTCTCCCCACAATCGAA3215528
Sprr2AFwd, 5′-CTGAGACTCAAGTACGATGTCTTACTACC; Rev, 5′-TTTTCTGTGAGGAGCCATCATAGGCAC; Probe, 5′-CCTTGTCCTCCTCCAAAGTG4476028
DNase IFwd, 5′-GATTTCCAAGCAGAATACGGACT; Rev, 5′-GAGATGGTGGAATGCTGTAGTACC; Probe, 5′-TGGCTGAAGCCATCAGTGACCATTA1756034
Siat4cFwd, 5′-ATAAAGAGCCTGGAGTGTCGTCG; Rev, 5′-TTTCCAGAAGCCTTTTCGCAC; Probe, 5′-GTCATCAACAAGTACGACGTG1756034
Daf1Fwd, 5′-ATGATCCGTGGGCGGGCGCCT; Rev, 5′-ATCTATGCACCGGGGTGGTGGAC; Probe, 5′-CAAGGAAAGGAGAGGAAGGATGTC8466028
RelmβFwd, 5′-AAGGAAGCTCTCAGTCGTCAA; Rev, 5′-GAGTCAGGTTTCCTGGTCGAG; Probe, 5′-CTGTGGATCGTGGGATATCCG2865528
Ckmt1Fwd, 5′-TGTGATCCAAGAGCGGCATAA; Rev, 5′-GCATCCACCACAACACGTTCTAC; Probe, 5′-GACCTTGATGCCAGTAAAATTCGTT2095832
  • a Fwd, forward; Rev, reverse.