Genes identified via SCOTS

ClassClone(s)Gene and/or possible functiona% Identity/ % similaritybSpancReference(s)dGenBank accession no.
SecretedH7ApxIVA var3 RTX toxin (A. pleuropneumoniae)33AAD01905
Cell surfaceB5Autotransporter adhesin, similar to Hsf in H. influenzae (A. pleuropneumoniae)29, 39, 41ZP_00204542
E4flpD, fimbria-like protein (H. ducreyi); flp genes are essential for microcolony formation in H. ducreyi65/6618812, 31, 37NP_873737
B9Outer membrane protein similar to immunogenic protein PomA of P. multocida (A. pleuropneumoniae)13, 42E47068
F4Outer membrane protein of unknown function (A. pleuropneumoniae)ZP_00135264
MetabolismA7guaA GMP synthase (A. pleuropneumoniae), linked to virulence in various organisms, including P. multocida12, 27, 32, 34ZP_00134556
A8Diadenosine tetraphosphatase similar to ApaH in S. enterica serovar Typhimurium (A. pleuropneumoniae); linked to virulence in H. parasuis and serovar Typhimurium21, 24ZP_00134055
A9fuc1, l-fucose isomerase (H. influenzae); in Bacteroides thetaiotaomicron, the l-fucose metabolic pathway is coordinated with the production of fucosylated glycans in enterocytes76/7822222, 25P44779
C2ptsB, sucrose-specific phosphotransferase EII component (P. multocida); linked to virulence in serovar Typhimurium, S. aureus, and H. influenzae83/8521628NP_246785
H3Sugar phosphate permease (A. pleuropneumoniae)ZP_00135166
C7Oxygen-sensitive ribonucleoside-triphosphate reductase (A. pleuropneumoniae)ZP_00134686
D2Thiol:disulfide interchange protein, similar to DsbA in H. influenzae (A. pleuropneumoniae); essential for virulence factor expression in P. aeruginosa17, 34ZP_00134426
D33-Polyprenyl-4-hydroxybenzoate decarboxylase (A. pleuropneumoniae)ZP_00134932
G6Ribosomal protein RpS19 (H. influenzae)100/10090P44385
E12Biotin-(acetyl-coenzyme A carboxylase) ligase (A. pleuropneumoniae)ZP_00134488
F12Dethiobiotin synthetase (A. pleuropneumoniae)ZP_00135280
F11NADH ubiquinone oxidoreductase subunit 5 (A. pleuropneumoniae)34ZP_00135039
G8SAM-dependent methyltransferase (A. pleuropneumoniae)34ZP_00134291
A4Uncharacterized conserved protein involved in oxidation of intracellular sulfur (A. pleuropneumoniae)ZP_00133817
A11Transposase (A. pleuropneumoniae)ZP_00134532
D13-Oxoacyl-(acyl-carrier protein) reductase (H. influenzae)78/82238P43713
D4Predicted GTPase (A. pleuropneumoniae)ZP_00134954
G11ATPase (A. pleuropneumoniae)ZP_00134371
E9Restriction-modification system (ALXA and HSDM, M . haemolytica); M. haemolytica AlxA activates leukotoxin transcription in E. coli81/8115620P95510
F8Molybdopterin biosynthesis protein (H. ducreyi)84/8617434AAP95073
H6Leucyl-tRNA synthetase (A. pleuropneumoniae)ZP_00133841
F9Predicted hydrolase of alkaline phosphatase superfamily (A. pleuropneumoniae)ZP_00134565
RegulatoryA3rpoB, RNA polymerase subunit (H. ducreyi)91/94207AAP96608
B2Cell shape-determining protein, similar to MreC (A. pleuropneumoniae)ZP_00134717
A10Signal recognition particle protein (H. influenzae)88/93243P44518
H5Molecular chaperone (A. pleuropneumoniae)ZP_00134922
H4Putative transcriptional regulator (A. pleuropneumoniae)ZP_00134590
StressC9tufA, elongation factor (EF) Tu (H. ducreyi); EF-Tu acts as a fibronectin binding protein in M. pneumoniae, linked to virulence in K. pneumoniae100/1001359, 40NP_872680
TransportA5apaA, putative ABC transporter, immunogenic (A. pleuropneumoniae)30AF109148
H1dmsA, dimethyl sulfoxide reductase, catalytic subunit (A. pleuropneumoniae); deletion mutants are attenuated2AY138463
C4hgbA, hemoglobin-binding protein (A. pleuropneumoniae); enables iron uptake from hemoglobin5, 38AAO33396
G1Na+/H+ antiporter (A. pleuropneumoniae); essential for in vivo survival in S. agalactiae27ZP_00134319
E1Predicted metal-binding protein (A. pleuropneumoniae)ZP_00135073
E7Membrane components of Kef-type potassium transport systems (A. pleuropneumoniae)ZP_00134994
F1Anaerobic C4-dicarboxylate transporter (A. pleuropneumoniae)34ZP_00134386
F2tbpA, transferrin receptor; small subunit (A. pleuropneumoniae); upregulated and essential in vivo1CAA90896
F5ATPase; cation transport (A. pleuropneumoniae)ZP_00135075
G10Di- and tricarboxylate transporters (A. pleuropneumoniae)ZP_00135062
UnknownB8Hypothetical protein PA2218 (P. aeruginosa)76/83162Q01609
G7, F7eA. pleuropneumoniae; no homology to known genes
  • a Genes with highest similarity to SCOTS sequences, obtained from public databases, source organism in parentheses, genes names given where available. SAM, S-adenosylmethionine.

  • b Identity/similarity percentages apply to amino acids.

  • c Span means the number of nucleotides over which similarity was detected.

  • d Publications reporting the isolation of identical or similar sequences.

  • e Sequences have not been submitted as these are partial open reading frames only.