Strains and plasmids used in the present study

Strain or plasmidRelevant characteristic(s)Reference or source
    M99S. gordonii endocarditis isolate23
    PS321M99 Δ[nss-gtf]; Ermr3
    PS436M99 gspB::pVA891 (nonpolar mutation); Ermr3
    ChallisaS. gordonii strain CH1D. Clewell
    PS779Challis Δhsa::pEVP3;CmrThis study
    PS787Challis hsa::pM99B194His6int; CmrThis study
    PS795Challis (pM99secA2); ErmrThis study
    PS796Challis Δ[nss-gtf];ErmrThis study
    PS797PS787 (pM99secA2); secretes HsaH6; Ermr CmrThis study
    PS798Challis secA2::pM993′A2; ErmrThis study
    PS799PS787 secA2::pM993′A2; secretes HsaH6; Ermr CmrThis study
    pVA891erm (gram positive), cat tet ori (E. coli)14
    pEVP3cat (gram positive and E. coli), ori (E. coli), lacZ6
    pMSP3535erm, ori (gram positive and E. coli), nisin-inducible promoter5
    pB194His6intSpeI-NheI fragment of gspB fused with six histidine codons and cloned in pEVP32
    pSgΔnss-gtfpVA891 and flanking chromosomal DNA rescued from PS321This study
    pSgΔBR5′ and 3′-end fragments of gspB in pEVP3This study
    pM993′A2Sau3AI-EcoRV fragment spanning the M99 secA2codon 557 through codon 22 of gtf cloned in pVA891This study
    pM99secA2secA2 of M99 in pMSP3535This study
  • a This strain, thought to be identical to DL1 and V288, may also be an endocarditis isolate (M. Vickerman and S. Gill [T1GR], personal communication).