TABLE 1.

Strains and plasmids used in the present study

Strain or plasmidRelevant characteristic(s)Reference or source
Strains
    M99S. gordonii endocarditis isolate23
    PS321M99 Δ[nss-gtf]; Ermr3
    PS436M99 gspB::pVA891 (nonpolar mutation); Ermr3
    ChallisaS. gordonii strain CH1D. Clewell
    PS779Challis Δhsa::pEVP3;CmrThis study
    PS787Challis hsa::pM99B194His6int; CmrThis study
    PS795Challis (pM99secA2); ErmrThis study
    PS796Challis Δ[nss-gtf];ErmrThis study
    PS797PS787 (pM99secA2); secretes HsaH6; Ermr CmrThis study
    PS798Challis secA2::pM993′A2; ErmrThis study
    PS799PS787 secA2::pM993′A2; secretes HsaH6; Ermr CmrThis study
Plasmids
    pBluescriptAmprStratagene
    pVA891erm (gram positive), cat tet ori (E. coli)14
    pEVP3cat (gram positive and E. coli), ori (E. coli), lacZ6
    pMSP3535erm, ori (gram positive and E. coli), nisin-inducible promoter5
    pB194His6intSpeI-NheI fragment of gspB fused with six histidine codons and cloned in pEVP32
    pSgΔnss-gtfpVA891 and flanking chromosomal DNA rescued from PS321This study
    pSgΔBR5′ and 3′-end fragments of gspB in pEVP3This study
    pM993′A2Sau3AI-EcoRV fragment spanning the M99 secA2codon 557 through codon 22 of gtf cloned in pVA891This study
    pM99secA2secA2 of M99 in pMSP3535This study
  • a This strain, thought to be identical to DL1 and V288, may also be an endocarditis isolate (M. Vickerman and S. Gill [T1GR], personal communication).