TABLE 3.

Expression of E. coli CFT073 class III genes of motility and chemotaxis

GeneFunctionFold changeaP value
flgL b Flagellar biosynthesis; hook-filament junction protein −3.19 0.054
flgK Flagellar biosynthesis, hook-filament junction protein 1 −2.36 0.004
fliD Flagellar biosynthesis; filament capping protein; enables filament assembly−1.310.018
fliS Flagellar biosynthesis; repressor of class 3a and 3b operons (RflA activity)−2.530.206
fliT Flagellar biosynthesis; repressor of class 3a and 3b operons (RflA activity)−1.040.441
fliC Flagellar biosynthesis; flagellin, filament structural protein −4.65 0.006
tar Methyl-accepting chemotaxis protein II, aspartate sensor receptor−3.880.060
cheZ Chemotactic response; CheY protein phophatase−3.490.107
cheY Transmits chemoreceptor signals to flagellar motor components−3.730.146
cheB Response regulator for chemotaxis (CheA sensor); protein methylesterase−0.890.397
cheR Response regulator for chemotaxis; protein glutamate methyltransferase−0.350.678
cheW Positive regulator of CheA protein activity −3.35 0.012
cheA Sensory transducer kinase between signal receptors and CheB and CheY−2.030.117
motB Enables flagellar motor rotation−1.360.186
motA Proton conductor component of motor−0.140.903
  • a Fold change refers to growth in vivo relative to growth in LB.

  • b Genes statistically downregulated in vivo at least two-fold are in boldface.