TABLE 2.

Comparison of innate and adaptive cell infiltrates to OD and CV tissues with differing inoculative doses

DoseODRatioCVRatio
No. of Innate cellsaNo. of Th1 cellsbNo. of Innate cellsaNo. of Th1 cellsb
1 × 107600 (74)40 (0.8)15390 (83)20 (16)20
1.5 × 105500 (73)36 (13)14400 (23)39 (10)10
1 × 104300 (61)37 (9.7)8.1230 (38)18 (18)13
  • a Number of PMN (103) and DC (103) per 106 GT cells (± standard errors of the means) 7 days postinfection. PMN and DC were identified in a pool of genital tract cells by flow cytometry, using LY6G and CD11c monoclonal antibodies, respectively. Data were compiled from three independent experiments.

  • b Number of Th1 cells (103) per 106 GT cells (± standard errors of the means) 14 days postinfection. Chlamydia-responsive Th1 cells were identified in a pool of genital tract cells using IFN-γ and IL-4 intracellular cytokine staining following a 48-h stimulation with Chlamydia antigen. Data were compiled from two independent experiments.