TABLE 2.

Primers

PrimerSequenceFunction
etrA-p1h1TAATATTTACCCTAAATGATTTTAGAGTACAGGTTGGCATGTGTAGGCTGGAGCTGCTTCPCR for mutagenesis
etrA-p2h2GTTATTATGATTTTACTATTATATCATTACTAATGACATACATATGAATATCCTCCTTAGPCR for mutagenesis
ygeH-p1h1GCTTATGCAGGATGCAAGAAACCAATTTTTTCATAGAGGTTAACTAGTGTAGGCTGGAGCTGCTTCGAAPCR for mutagenesis
ygeH-p2h2AACAAGTTGCATACATCTAAATAATAAAATTGTGTCAATAAAAATTTACATATGAATATCCTCCTTAGTTCCTPCR for mutagenesis
eivF-p1h1AGTTTATGGAGAATGAGAATATGGAATAAGGAGATGAAACGTGTAGGCTGGAGCTGCTTCPCR for mutagenesis
eivF-p2h2AAGACTGAAATTAATATAATAGTAATGCGTAATTTTATTTCATATGAATATCCTCCTTAGPCR for mutagenesis
YgeHfor1AGCGCAGGCATATATTGAACTCTConfirmation of mutagenesis
YgeHrev1GAAAAGACGCCATCCATGTTGAAConfirmation of mutagenesis
EtrAfor1TTCCCTCAACGTTAAGCAAATATConfirmation of mutagenesis
EtrArev1ACCTGTGCAAATTCGCGATATConfirmation of mutagenesis
EivFfor1ATGCGGGGAGTAACAAAACACConfirmation of mutagenesis
EivFrev1CAGGCGTTCAATCAAGGCATConfirmation of mutagenesis
Bad-etrA-forCCCTTCTAGATTTTAGAGTACAGGTTGGCAComplementation
Bad-etrA-revCTAATAAGCTTTCAACTTTCTCTTACGCAAGATTGGComplementation
Bad-eivF-forAGAATCTAGAATAAGGAGATGAAACATGAComplementation
Bad-eivF-revGCATAAGCTTGAAATTAATATAATAGTAATGCGTAATTTTATTTComplementation
Bad-forCTGTTTCTCCATACCCGTTInsert screening
Bad-revCTCATCCGCCAAAACAGInsert screening