TABLE 3.

Summary of rCPE variant phenotypes

rCPE speciesConcn needed for:Small complex formationLarge complex formation
50% maximal 86Rb release (μg/ml)50% [125I]CPE binding inhibition (μg/ml)
rCPE0.57 ± 0.193.69 ± 0.52++
D45A0.24 ± 0.041.83 ± 0.54++
K46A0.82 ± 0.072.89 ± 0.86++
G47A0.92 ± 0.635.52 ± 0.52++
D48A>503.31 ± 0.78+
G49A0.83 ± 0.232.45 ± 0.59++
W50A1.35 ± 0.644.13 ± 0.90++a
I51A>502.59 ± 0.16+
L52A0.68 ± 0.211.45 ± 0.04++
G53A0.52 ± 0.153.20 ± 0.74++
E54A0.38 ± 0.192.47 ± 0.56++
D48E>501.92 ± 0.05+
D48N>502.21 ± 0.16+
W50Y0.65 ± 0.42.68 ± 0.45++
W50F0.9 ± 0.22.60 ± 1.41++
I51L0.9 ± 0.33.23 ± 0.18++
I51V1.6 ± 1.22.82 ± 0.57++
  • a The W50A variant produces an atypical large complex (Fig. 6).