TABLE 1.

Characteristics of genes in mel locia

Locus and geneM. tuberculosis homologue (% identity, no. of aa)bE value (score)cDeduced length (aa)Other similarity or motif(s)dPutative activity
mel1
    melARv2560 (63, 249)8e-79 (296)377CO65473, integral membrane protein; proline-rich amino terminus; gram-positive bacterial signal sequenceMembrane protein (unknown)
    melBRv2566 (78, 1,121)0.0 (1,730)1,106CO61305, cysteine protease-like transglutaminases; gram-positive bacterial signal sequenceTransglutaminase or protease
    melCRv2567 (77, 894)0.0 (1,311)896COG2307, COG2308, uncharacterized conserved protein, gram-positive bacterial signal sequenceUnknown
    melDRv2568c (80, 336)1e-159 (563)350COG4307, uncharacterized conserved proteinUnknown
    melERv2569c (84, 282)1e-134 (478)336COG1305, cysteine protease-like transglutaminases, gram-positive bacterial signal sequenceTransglutaminase or protease
mel2
    melFRv1936 (95, 365)0.0 (687)379COG2141, flavin-dependent oxidoreductases and luciferase-like monooxygenase, luxA, alkanal monooxygenaseOxidoreductase
    melGRv1937 (83, 837)0.0 (1,434)839Fer2, 2Fe-2S, NqrF, UbiH, dioxygenase, dehydrogenase, luxGDehydrogenase
    melHRv1938, ephB (82, 355)1e-173 (608)352KOG4178, epoxide hydrolase, luxHLipid transport and metabolism
    melIRv1939 (78, 170)5e-71 (267)177COG1853, oxidoreductaseFlavoprotein oxygenase
    melJRv1940, ribA (68, 344)1e-116 (419)365KOG1284, bifunctional GTP cyclohydrolase II-DHBP synthaseCyclohydrolase
    melKRv1941 (78, 221)3e-77 (290)256KOG0725, broad-specificity-range reductase, fabGLipid metabolism-synthesis
  • a Abbreviations: aa, amino acids; COG, cluster of orthologous groups; KOG, cluster of orthologous groups for eukaryotic genome; Fer2, 2Fe-2S, iron-sulfur cluster binding domain; NqrF, Na+-transporting NADH:ubiquinone oxidoreductase; UbiH, 2-polyprenyl-6-methoxyphenol hydroxylase; DHBP, 3,4-dihydroxy-2-butanone-4-phosphate.

  • b M. tuberculosis gene to which the M. marinum gene displays similarity, along with percent identity at amino acid level and number of amino acids that could be aligned to show this level of identity.

  • c Expectation frequency (E value) and score according to NCBI protein-protein BLAST in comparison with M. tuberculosis homologue.

  • d Placement of putative protein product into a cluster of orthologues (COG or KOG) by use of CD-Search at NCBI and SignalP 3.0 at the Center for Biological Sequence Analysis Website.