TABLE 1.

Primer sequences of oligonucleotides used for cloning, sequence analysis, and serovar classification

Category of use and primerOligonucleotide sequence (5′ → 3′)Reference
Cloning and amplification for sequencing
    00.12GCT TTC ATC CAC ATC TTT GAC CGThis study
    JHC250CCC GTT GCA GAG CGG ATGThis study
    JHC274CTC CAT CGC ATC ATA CAG GThis study
    JHC275CCT GTA TGA TGC GAT GGA GThis study
    JHC450CGC GGA TTA GAT CCT TAA GAG TGGThis study
    JHC451GGA GCG CCG CTT CTA CAG AAG GAA AGThis study
Serovar classification
    JHC202ATG AAA AAA CTC TTG AAA TCG GTAThis study
    JHC203TTA GAA GCG GAA TTG TGC ATT TACThis study
    JHC440GAG CTG CAA TCT ATG AAG CAA GCT CThis study
    JHC441GCT CCA TAG CAC CTT TAA TAT GCC CThis study
    JHC442TGG TCA GAT CGA CGA GTC CGA GAT CThis study
    JHC443CAT TTC TTT TGA ACT TAC GCA GCA TGA GThis study