TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or description and originSource or reference(s)
E. coli strains
    HB101F thi-1 hsdS20(rB mB) supE44 recA13 ara-14 leuB6 proA2 lacY1 galK2 rpsL20 (strR) xyl-5 mtl-1Promega
    TOP10F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (strR) endA1 nupGInvitrogen
H. ducreyi strains
    35000HPClass I clinical isolate; human-passaged variant of strain 35000, Winnipeg, Canada, 19752, 21, 51
    HD183Class I clinical isolate; Singapore, 19829, 48
    HD188Class I clinical isolate; Kenya, 19829, 48
    82-029362Class I clinical isolate; California, 19829, 48
    6644Class I clinical isolate; Massachusetts, 19899, 48
    HD85-023233Class I clinical isolate; New York, 19859, 48
    CIP542-ATCCClass II clinical isolate; Hanoi, Viet Nam, 195421, 51
    HMC112Class II clinical isolate; 1984 (location not reported)51
    33921Class II clinical isolate; Kenya (yr not reported)9, 13
    DMC164Class II clinical isolate; Bangladesh (yr not reported)51
    MEB10735000HPsapA::kanThis study
    MEB12135000HPsapA::kan/psapAThis study
Plasmids
    pGEM-T EasyTA cloning vector; AmpraPromega
    pSC-ATA cloning vector; AmprStratagene
    pUC18K2Source of nonpolar Kanr cassette37
    pRSM2072H. ducreyi suicide vector12
    pLSSKH. ducreyi shuttle vector52
    pMEB0785′ end of sapA in pGEM-T EasyThis study
    pMEB0803′ end of sapA in pGEM-T EasyThis study
    pMEB0925′ end of sapA + Kanr cassette in pGEM-T EasyThis study
    pMEB0985′ end of sapA + Kanr cassette + 3′ end of sapA in pGEM-T EasyThis study
    pMEB108sapA ORF in pGEM-T-EasyThis study
    pMEB114Putative sap promoter in pSC-AThis study
    pMEB115Putative sap promoter + sapA ORF in pGEM-T EasyThis study
    pMEB120Putative sap promoter + sapA ORF in pLSSKThis study
  • a Ampr denotes resistance to ampicillin.