TABLE 1.

P. aeruginosa strains and plasmids used in this study

Strain or plasmidRelevant characteristicsReference and/or source
P. aeruginosa strains
    PA99Clinical isolate; naturally secretes ExoS, ExoT, and ExoU8
    PA99nullSecretes no effectors; PA99 ΔexoS ΔexoT ΔexoU; gentamicin resistant37
    PA99secrSecretes no effectors; does not make secretion apparatus; PA99 ΔpscJ37
    PA99SSecretes only ExoS36
    PA99STSecretes ExoS and ExoT36
    PA99null+ExoUPA99null complemented with exoU in the attB locusThis study
    PA99null+ExoU(S142A)PA99null complemented with exoU(S142A) in the attB locus6
    PA99null+ExoU-BlaPA99null complemented with exoU-bla in the attB locusThis study
    PA99null+ExoU(S142A)-BlaPA99null complemented with exoU(S142A)-bla in the attB locusThis study
    PA99null+GST-BlaPA99null complemented with gst-bla under the control of native exoU promoter in the attB locusThis study
    PA99secr+ExoU-BlaPA99secr complemented with exoU-bla in the attB locusThis study
    PA99secr+ExoU(S142A)-BlaPA99secr complemented with exoU(S142A)-bla in the attB locusThis study
    PA99S+ExoU(S142A)-BlaPA99S complemented with exoU(S142A)-bla in the attB locusThis study
    PA99ST+ExoU(S142A)-BlaPA99ST complemented with exoU(S142A)-bla in the attB locusThis study
Plasmids
    mini-CTX-1Plasmid for chromosomal gene integration into attB locus; TetrH. Schweizer (16)
    mini-CTXexoUexoU gene ligated into mini-CTX-1; Tetr29
    mini-CTXexoU-blamini-CTXexoU with C-terminal bla tag; TetrThis study
    mini-CTXexoU(S142A)-blamini-CTXexoU(S142A) with C-terminal bla tag; TetrThis study
    mini-CTXgst-blagst-bla replaces exoU coding sequence in mini-CTXexoU-bla; TetrThis study