TABLE 1.

Bacterial strains and plasmids

Strain or plasmidGenotype or descriptionSource or reference
E. coli SM10λpirthi thr leu tonA lacY supE recA::RP4-2-Tc::Mu (λpirR6K) Kmr21
V. cholerae strains
    PW357MO10 lacZ::vpsLplacZ Smr8
    PW751MO10 lacZ::vpsLplacZ ΔPTS Smr12
    PW836MO10 lacZ::vpsLplacZ ΔEIIAGlc Smr12
    PW961MO10 lacZ::vpsLplacZ ΔEI Smr12
    PW964MO10 lacZ::vpsLplacZ ΔHPr Smr12
MO10 lacZ::vpsLplacZ ΔFPr SmrThis study
MO10 lacZ::vpsLplacZ ΔNPr SmrThis study
    PW965MO10 lacZ::vpsLplacZ ΔHPr ΔFPr SmrThis study
MO10 lacZ::vpsLplacZ ΔHPr ΔNPr SmrThis study
MO10 lacZ::vpsLplacZ ΔPTS ΔFPr SmrThis study
MO10 lacZ::vpsLplacZ ΔEIIANtr1 SmrThis study
MO10 lacZ::vpsLplacZ ΔEIIANtr2 SmrThis study
MO10 lacZ::vpsLplacZ VC0672::pGP704 Smr AprThis study
MO10 lacZ::vpsLplacZ VCA1045::pGP704 Smr AprThis study
MO10 lacZ::vpsLplacZ VCA0245::pGP704 Smr AprThis study
MO10 lacZ::vpsLplacZ VC1820::pGP704 Smr AprThis study
MO10 lacZ::vpsLplacZ VC1822::pGP704 Smr AprThis study
MO10 lacZ::vpsLplacZ VC1826::pGP704 Smr AprThis study
    PW249MO10This study
MO10 ΔEI SmrThis study
MO10 ΔEI ΔvpsL SmrThis study
Plasmids for construction of deletions
    pWM91oriR6K mobRP4 lacI pTac tnp mini-Tn10(Km) Kmr Apr20
    pWM91ΔPTSpWM91 carrying an unmarked, in-frame deletion of the PTS operon12
    pWM91ΔEIIAGlcpWM91 carrying an unmarked, in-frame deletion in VC096412
    pWM91ΔEIpWM91 carrying an unmarked, in-frame deletion in VC096512
    pWM91ΔHPrpWM91 carrying an unmarked, in-frame deletion in VC096612
    pWM91ΔNPrpWM91 carrying an unmarked, in-frame deletion in VC2533This study
    pWM91ΔFPrpWM91 carrying an unmarked, in-frame deletion in VCA0518This study
    pWM91ΔEIIANtr1pWM91 carrying an unmarked, in-frame deletion in VC2531This study
    pWM91ΔEIIANtr2pWM91 carrying an unmarked, in-frame deletion in VC1824This study
Plasmids for construction of insertions
    pGP704::VC1820pGP704 carrying an internal fragment of VC1820This study
    pGP704::VC1822pGP704 carrying an internal fragment of VC1822This study
    pGP704::VC1826pGP704 carrying an internal fragment of VC1826This study
    pGP704::VCA1045pGP704 carrying an internal fragment of VCA1045This study
    pGP704::VCA0245pGP704 carrying an internal fragment of VCA0245This study
    pGP704::VC0672pGP704 carrying an internal fragment of VC0672This study
    pGP704::VC1283pGP704 carrying an internal fragment of VC1283This study
Plasmids used in rescue experiments
    pBAD-TOPO-EIpBAD-TOPO carrying the coding sequence of VC096512
    pBAD-TOPO-HPrpBAD-TOPO carrying the coding sequence of VC096612
    pBAD-TOPO-HPr(H15A)pBAD-TOPO carrying a VC0966 variant encoding an H-to-A mutation at position 15This study
    pBAD-TOPO-FPrpBAD-TOPO carrying the coding sequence of VCA0518This study
    pBAD-TOPO-FPr(H324A)pBAD-TOPO carrying a VCA0518 variant encoding an H-to-A mutation at position 324This study
    pBAD-TOPO-FPr(309-401)pBAD-TOPO carrying a fragment of VCA0518 encoding positions 309 to 401This study