TABLE 1.

Strains and plasmids used in this study

Strain or plasmidCollection site or relevant genotypePFGE typeaSerum growthbSourcec or reference
A. baumannii
    510SputumAIUNMC (NE; 2006)
    2824UrineAUNMC (NE; 2008)
    2898Ankle woundAUNMC (NE; 2008)
    4564UrineAUNMC (NE; 2006)
    4860UrineAUNMC (NE; 2007)
    4884UrineAUNMC (NE; 2007)
    4893Leg woundAUNMC (NE; 2007)
    5191UrineAUNMC (NE; 2007)
    5393UnknownAUNMC (NE; 2007)
    5562BloodAIUNMC (NE; 2007)
    5592SputumAUNMC (NE; 2007)
    5695UnknownAUNMC (NE; 2007)
    ATCC 17904UrineBRATCC (1963)
    ATCC 17961BloodCRATCC (1968)
    ATCC 17976Postoperative meningitisDIATCC (1951)
    ATCC 17978Fetal meningitisEIATCC (1951)
    98-37-01Endotracheal tubeFRCDC (TX; 1998)
    98-37-02SputumGRCDC (TX; 1998)
    98-37-05Tracheal aspirateHICDC (TX; 1998)
    98-37-09Cerebrospinal fluidIRCDC (TX; 1998)
    ACJ1EZ-Tn5::A1S_3365 derivative of 98-37-09IIThis work
    ACJ2EZ-Tn5::A1S_2989 derivative of 98-37-09IIThis work
    ACJ3EZ-Tn5::A1S_0269 derivative of 98-37-09IIThis work
    ACJ4EZ-Tn5::A1S_3045 (promoter region) derivative of 98-37-09IIThis work
    ACJ5EZ-Tn5::A1S_3045 (promoter region) derivative of 98-37-10IIThis work
    ACJ6EZ-Tn5::A1S_3278 derivative of 98-37-09IIThis work
    98-37-11Bronchial washJRCDC (TX; 1998)
    01-12-05BloodKRCDC (IN; 2001)
    01-12-06SputumKCDC (IN; 2001)
    01-12-07SputumKCDC (IN; 2001)
    01-12-08Burn woundKCDC (IN; 2001)
    01-12-09Catheter tipKCDC (IN; 2001)
    07-09-54UnknownLICDC (KY; 2007)
    07-09-59UnknownLCDC (KY; 2007)
    07-09-60UnknownLCDC (KY; 2007)
    07-09-61UnknownLCDC (KY; 2007)
    07-09-63UnknownLCDC (KY; 2007)
E. coli
    INVαF′F′ endA1 recA1 hsdR17 (rK mK+) supE44 thi-1 gyrA96 relA1 φlacZΔM15 Δ(lacZYA-argF)U169 λInvitrogen
Plasmids
    pCR II-TOPOAmpr KanrInvitrogen
    pWH1266Ampr Tetr15
    pACJ01pWH1266/A1S_2989 TetrThis work
  • a PFGE pattern of the isolate in comparison to all other isolates in the study.

  • b Human serum growth/resistance profile relative to all other PFGE types assessed; I and R indicate intermediate and robust growth, respectively.

  • c Listed are the strain source with the location and/or year of isolation in parentheses. UNMC, University of Nebraska Medical Center.