TABLE 1.

B. burgdorferi strains used in this study

StrainDescriptionReference
B314Attenuated B. burgdorferi strain B31 clone lacking all linear plasmids, including lp54 46
B31-A34Transformable, noninfectious B31 clone lacking lp5, lp25, lp28-1, lp28-4, lp56, lp36, cp-9, and cp32-6, used for testing the Cre-lox system 26
B31-A3Transformable, infectious B31 clone lacking cp9; background of lp54 mutants listed below 15
A3-A07Streptomycin resistance cassette and loxP insertion into bba07This study
A3-A01/A07Streptomycin resistance cassette and loxP insertion into bba07, kanamycin resistance cassette and loxP insertion into bba01This study
A3-A07/A14Streptomycin resistance cassette and loxP insertion into bba07, kanamycin resistance cassette and loxP insertion into bba14This study
A3ΔA1-7Deletion of bba01 to bba07 loci and retention of only the streptomycin resistance cassetteThis study
A3ΔA7-14Deletion of bba07 to bba14 loci and retention of only the kanamycin resistance cassetteThis study