TABLE 1.

E. coli strains and plasmids

Strain or plasmidGenotype or descriptionaReference or source
Strains
    BW19795RP4-2-tet::Mu-1kan::Tn7 integrant/srlC300 creC510 hsdR17 endA1 zbf-5 uidA(ΔMluI)::pir+thiG. Phillips
    DH5αF φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK mK+) phoA supE44 λthi-1 gyrA96 relA1Invitrogen
    ISM1536 E. coli O157:H7 EDL933N. Cornick
    ISM1191 E. coli O157:H7 EDL933, NalrThis study
    ISM1205BW19795 pUTminiTn5Km2; Ampr Kanr 40
    ISM1230 E. coli O157:H7 EDL933 lacking plasmid pO157N. Cornick
    ISM1893ISM1191/miniTn5Km2 in hlyB, Nalr KanrThis study
    ISM2014ISM1536 ΔhlyA KanrThis study
    ISM1211ISM1536 ΔhlyB KanrThis study
    ISM1216ISM1536 ΔhlyD KanrThis study
    ISM2013ISM1216/pISM30 Ampr KanrThis study
    ISM1978ISM1536/pISM31 SpcrThis study
    ISM2015ISM1230/pISM31 SpcrThis study
    ISM1944ISM1893/pISM31 Spcr KanrThis study
    ISM2016ISM 2014/pISM31 Spcr KanrThis study
    ISM2017ISM1211/pISM31 Spcr KanrThis study
    ISM2018ISM1216/pISM31 Spcr KanrThis study
    ISM2019ISM2013/pISM31 Spcr KanrThis study
    ISM1881ISM1191/miniTn5Km2 in L7020, Nalr KanrThis study
    ISM1919ISM1191/miniTn5Km2 in hlyC hlyA, Nalr KanrThis study
    ISM1227ISM1536 ΔL7020 KanrThis study
Plasmids
    pKD4Derivative of pANTSγ that contains an FLP recombination target-flanked Kanr gene from pCP15 13
    pRedETDerivative of pSC101, Ampr, temp sensitive, carries lambda red recombinaseGene Bridges
    pBAD18Expression vector, AmprBeckwith lab
    pISM30pBAD18 expressing ehxCABD operonThis study
    pISM31pMHE6 expressing GFPuvG. Phillips
  • a Nalr, nalidixic acid resistant; Ampr, ampicillin resistant; Kanr, kanamycin resistant; Spcr, spectinomycin resistant.