TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaReference or source
Strains
    197NParental B. avium strain; Strs Nalr Kms Hag+33
    197N2197N; spontaneous Strr mutantThis study
    P206b197N except hagA::TnphoA206 Kmr HagThis studyb
    P207a197N except hagA::TnphoA207 Kmr HagThis study
    G145197N except hagA::TnlacZ145 Kmr HagThis studyc
    P225a197N except hagA::TnphoA225 Kmr HagThis study
    P218a197N except hagA::TnphoA218 Kmr HagThis study
    P215b197N except hagA::TnphoA215 Kmr HagThis study
    P208a197N except hagB::TnphoA208 Kmr HagThis study
    P212a197N except hagB::TnphoA212 Kmr HagThis study
    P205a197N except hagB::TnphoA205 Kmr HagThis study
    P201a197N except hagB::TnphoA201 Kmr HagThis study
    PAS666197N2 except ΔhagA Kms HagThis study
    PAS667197N2 except ΔhagB Kms HagThis study
    S17.1 λ pirE. coli conjugation donor; Strr Nals Kms9
    HB101E. coli cloning strainLaboratory collection
    DH5αE. coli cloning strainInvitrogen
    M13/pREP4E. coli cloning strain containing plasmid pREP4Qiagen
Plasmids
    pLAFR5Broad-host-range cloning vector16
    pUC19E. coli cloning vector36
    pCR2.1TOPOE. coli cloning plasmid; Kmr AprInvitrogen
    pQE30His tag cloning vectorQiagen
    pQE80-LHis tag cloning vectorQiagen
    pKAS46oriR6K oriT rpsL Apr Kmr30
    phagApLAFR5 hagAThis study
    phagBpLAFR5 hagBThis study
    pΔhagApTOPO ΔhagAThis study
    pΔhagBpTOPO ΔhagBThis study
    pKASΔhagApKAS46 ΔhagA KmrThis study
    pKASΔhagBpKAS46 ΔhagB KmrThis study
    pΩhagAhispQE30 ΩhagAhisThis study
    pΩhagBhispQE80-L ΩhagBhisThis study
  • a Abbreviations: Hag, phenotypic characteristic of hemagglutination; Strr, streptomycin resistant; Nalr, nalidixic acid resistant; Nals, nalidixic acid sensitive; Kms, kanamycin sensitive; Kmr, kanamycin resistant; Apr, ampicillin resistant. Sequences for each hagA and hagB mutant allele are available at GenBank, with accession numbers assigned as follows: hagA::TnphoA206, FJ001329; hagA::TnphoA207, FJ001327; hagA::TnlacZ145, FJ001328; hagA::TnphoA225, FJ001330; hagA::TnphoA218, FJ001331; hagA::TnphoA215, FJ001332; hagB::TnphoA208, FJ001333; hagB::TnphoA212, FJ001334; hagB::TnphoA205, FJ001335; hagB::TnphoA201, FJ001336; ΔhagA, GQ240879; and ΔhagB, GQ240878.

  • b This strain has been previously reported (33), but the map location of the lesion conferring the Hag phenotype was not known.

  • c The strain has been previously reported (28), but the map location of the lesion conferring the Hag phenotype was not known.