TABLE 2.

Oligonucleotides used in this study

PrimerDesignationSequencea
1FlaBp-loxP 5′ATAACTTCGTATAGCATACATTATACGAAGTTATtgtctgtcgcctcttgtggc
2GFP-loxP 3′ PstI ctgcagATAACTTCGTATAGCATACATTATACGAAGTTATttatttgtagagctcatcca
3Cre 5′ NdeI catatgtccaatttactgaccgtac
4Cre 3′ XbaI tctagactaatcgccatcttccagcagg
5229F ggtaccgattaatccaagctcaataa
61228Rggctcaatattgcgtttaag
7LoxflgF-1 ggatccATAACTTCGTATAGCATACATTATACGAAGTTATtacccgagcttcaaggaaga
8KanR-1 ggatccttagaaaaactcatcgagct
94297F ggtaccggcttgcagctgccttaata
105391Rtaaaagctgtagtcaagccc
11LoxflaBF gagctcATAACTTCGTATAGCATACATTATACGAAGTTATctgtcgcctcttgtggcttc
12aadaR gagctcttatttgccgactaccttgg
138398F acgcgtcaactctggcattgacgctt
149198Raaaaaagtcccagttttggg
15LoxflgF-2 aagcttATAACTTCGTATAGCATACATTATACGAAGTTATtacccgagcttcaaggaaga
16KanR-2 aagcttttagaaaaactcatcgagct
17pBSV2* 5′ttttattatagagttcatatacatgtttttctaaatctataa
18pBSV2* 3′acatgtatatgagttctataataaaaaatattctagtgaggg
19pncA+RBS EcoRI 5′ccggaattcttgtatcaagggatattgcc
20pncA XbaI 3′gctctagattatatattaagcttactttggctg
21Cre+RBS XbaI 5′gctctagataagaacaataaaaaggaaaatttatgtccaatttactgaccgtacacc
22cre SphI 3′acatgcatgcctaatcgccatcttccagcagg
23pncA prom 5′ EcoRIcggaattcctatgctatccccttgttcaagtc
24pncA prom 3′ EcoRIcggaattccttttgtcaataattatatatttggg
  • a Restriction enzyme sites are indicated in boldface type, and the loxP sequences are in uppercase type.