TABLE 3.

Mini-Tn5 transposon biofilm-negative mutants of E. coli O157:H7 EDL933e

MutantLocus tagGene nameProduct or function
Bnp1aZ3917bHypothetical protein
Bnp2aZ4881bPutative aldolase
Bnp3aZ5856Putative aspartate carbamoyltransferase
Bnp4aZ2256bUnknown protein associated with Rhs element
Bnp5aZ5890bPartial putative integrase
Bnp6aL7020d espP Putative exoprotein precursor
Bnp7aZ3635Putative receptor protein
Bnp8aZ3182b hisD l-histidinal:NAD+ oxidoreductase
Bnp9aZ4625 acrE Protein affects cell membrane permeability
Bnp10Z0472 yaiH Putative enzyme
Bnp11aZ1555bHypothetical protein
Bnp12aZ2436 ynaJ Hypothetical protein
Bnp13aZ1921cUnknown protein encoded by CP933X
Bnp14aZ0151 ecpD Putative fimbrial chaperone protein
Bnp15aZ1456c/Z1457cHypothetical protein encoded by BP-933W/putative DNA binding protein of BP-933W
Bnp16Z3592 aroC Chorismate synthase
Bnp17Z2026 cls Cardiolipin synthase
Bnp18aL7049d hlyB Hemolysin transport protein
Bnp19aZ3497 b glpQ Glycerophosphodiester phosphodiesterase
Bnp20Z4099 relA (p)ppGpp synthetase I
Bnp21Z5049 waaL Surface polymer ligase
Bnp22aZ3199b/Z3200b wbdP/per Putative glycosyl transferase/perosamine synthetase
Bnp23Z5050b waaD LPS biosynthesis enzyme
Bnp24aZ2163 ydeH Hypothetical protein
Bnp25Z1675/Z1676 csgB/csgA Minor curlin subunit precursor/curlin major subunit, coiled surface structures
Bnp26aZ2086cPutative regulator
Bnp27aZ3660 yfeA Hypothetical protein
Bnp28aZ5214bHypothetical protein
Bnp29Z5051b waaJ LPS α1,2-glucosyltransferase
Bnp30Z1670 csgG Curli production assembly/transport component
Bnp31aZ1212b/Z1213bHypothetical protein
Bnp32aZ1213bHypothetical protein
Bnp33Z2012/Z2013 galU/hns Glucose-1-phosphate uridylyltransferase/DNA binding protein; pleiotropic regulator
Bnp34Z3195/Z3196 manC/wbdQ Mannose-1-phosphate guanosyltransferase/GDP-mannose mannosylhydrolase
Bnp35aZ0340cUnknown protein encoded in prophage CP933I
Bnp36Z1676 csgA Curlin major subunit, coiled surface structures
Bnp37Z0905 tolQ Inner membrane protein
Bnp38Z5054 waaP LPS biosynthesis enzyme
Bnp39aZ4328bHypothetical protein
Bnp40aZ0700bPutative receptor
Bnp41aZ3918bPutative chaperone protein
Bnp42aZ1494cUnknown protein encoded by BP-933W
Bnp43aZ0021bHypothetical protein
Bnp44aL7047/L7048d hlyC/hlyA Hemolysin protein
Bnp45aZ1977 ychM Hypothetical protein
Bnp46aZ0408/Z0409 yahE/yahF Hypothetical protein/putative oxidoreductase subunit
Bnp47aZ3197 fcl Fucose synthetase
Bnp48aZ0904 ybgC Hypothetical protein
Bnp49aZ4327bHypothetical protein
Bnp50Z5392 dsbA Protein disulfide isomerase I
Bnp51aZ1177b/Z1178bPartial putative phage inhibition protein/putative receptor
  • a Genes not previously shown to be involved with biofilm formation.

  • b On O islands.

  • c Carried in prophage.

  • d On pO157.

  • e A slash indicates that the transposon site was between the genes indicated.