TABLE 1.

Strains and plasmids used in this study

StrainPlasmidDescriptionSource or reference
P. intermedia 17Parent strainKai Leung, U.S. Army Dental Research Detachment, Great Lakes, IL
E. coli
    One ShotChemically competent cellInvitrogen
    TOP10pCR 2.1Cloning vectorInvitrogen
    V2920pVA2920Knr; pCR2.1 containing the 0.9-kb adpC gene in TOP10 cellsThis study
    V2846pVA2846Knr; pET30a containing the 0.9-kb adpC gene in BL21(DE3) cells; expression constructThis study
    V2720pVA2720Knr; pET30aNovagen
    V2848pVA2848Knr; pET30a containing the 0.9-kb PI1571 gene in BL21(DE3) cells; expression constructThis study
    V2847pVA2847Knr; pET30a containing 0.9-kb PI2109 in BL21(DE3) cells; expression constructThis study
    V2921pVA2921Knr; pET30a containing the 2-kb C-terminal region of pPI0035 in BL21(DE3) cells; expression constructThis study
    V2922pVA2922Knr; pET30a containing 2-kb pPI0626 in BL21(DE3) cells; expression constructThis study