TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)aDerivation, source, or reference(s)
Strains
    E. coli
        BL21(DE3)F ompT hsdSB (rB mB) gal dcm (DE3)Novagen
        Top10F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
        χ289W1485 λ glnV44 F13
        χ6212F λ φ80 Δ(lacZYA-argF) endA1 recA1 hsdR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA455
        χ7213thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 ΔasdA4 recA1 RP4 2-Tc::Mu [λpir]; Kmr68
    S. enterica serovar Typhimurium
        χ3761Wild-type UK-118
        χ8914χ3761 ΔpabA1516 ΔpabB232 ΔasdA1692
        χ8916χ3761 ΔphoP233 ΔasdA1640
        χ9017χ8914 ΔrelA196::araC PBADlacI TTχ8914
        χ9018χ8916 ΔrelA196::araC PBADlacI TTχ8916
        χ9095χ9017 ΔaraBAD23χ9017
        χ9097χ8914 ΔaraBAD23χ8914
        χ9099χ8914 ΔrelA197::araC PBADlacI TTχ8914
        χ9101χ9099 ΔaraBAD23χ9099
        χ9241ΔpabA1516 ΔpabB232 ΔasdA16 ΔaraBAD23 ΔrelA198::araC PBADlacI TTχ9097
    S. pneumoniae
        A66.1Encapsulated type 34
        D39Encapsulated type 24
        E134Encapsulated type 239
        L82016Encapsulated type 6B9
        Tigr4Encapsulated type 483
        WU2Encapsulated type 310
    Yersinia pestis
        KIM6+Pgm+; pMT1 pPCP1; cured of pCD15
Plasmids
    pBAD-HisCHis-tag plasmid; Ap+Invitrogen
    pCR-BluntII-TopoTopo cloning vector; Ap+Invitrogen
    pET-28aExpression vector; Km+Novagen
    pGEM3ZCloning vector; Ap+Promega
    pMEG-375sacRB mobRP4 oriR6K; Cmr Apr22, 92
    pRE112sacB mobRP4 oriR6K, Cmr23
    pYA3342Asd+; pBR ori38
    pYA3493pYA3342 derivative periplasmic secretion plasmid based on β-lactamase N-terminal signal sequence38
    pYA3599pMEG375-based suicide vector to generate ΔaraBAD23 mutation; Cmr17
    pYA3620pYA3493 derivative periplasmic secretion plasmid based on β-lactamase N-terminal signal sequence and C-terminal sequence19
    pYA3624p15A ori; Asd+; araC PBAD cassette17
    pYA3627pYA3342 with Salmonella serovar Typhimurium lpp signal sequenceThis study
    pYA3638pYA3342 with Y. pestis psaA signal sequenceThis study
    pYA3698pGEM-3Z with T4 ipIII transcription terminator; AprThis study
    pYA3699pGEM-3Z with araC PBAD cassette; AprThis study
    pYA3700araC PBAD TT cassette plasmid; AprThis study
    pYA3751570-bp DNA encoding PsaA from aa 21 to aa 210 in pBAD-HisC for the expression of His-tag PsaA (aa 21 to 210)This study
    pYA3752570-bp DNA encoding PsaA from aa 21 to aa 210 in pYA3493This study
    pYA3753570-bp DNA encoding PsaA from aa 21 to aa 210 in pYA3620This study
    pYA3755573-bp DNA encoding PsaA from aa 20 to aa 210 in pBAD-HisC for the expression of His-tag PsaA(aa 20-210)This study
    pYA3756573-bp DNA encoding PsaA from aa 20 to aa 210 in pYA3493This study
    pYA3757573-bp DNA encoding PsaA from aa 20 to aa 210 in pYA3620This study
    pYA3759579-bp DNA encoding PsaA from aa 17 to 19 and aa 21 to 210 in pBAD-HisC for the expression of His-tag PsaAThis study
    pYA3760579-bp DNA encoding PsaA from aa 17 to 19 and aa 21 to 210 in pYA3493This study
    pYA3761579-bp DNA encoding PsaA from aa 17 to 19 and aa 21 to 210 in pYA3620This study
    pYA3763582-bp DNA encoding PsaA from aa 17 to aa 210 in pBAD-HisC for the expression of His-tag PsaA (aa 17 to 210)This study
    pYA3764582-bp DNA encoding PsaA from aa 17 to aa 210 in pYA3493This study
    pYA3765582-bp DNA encoding PsaA from aa 17 to aa 210 in pYA3620This study
    pYA3784pRE112-derived suicide vector for ΔrelA196::araC PBADlacI TT; CmrThis study
    pYA3879pRE112-derived suicide vector for ΔrelA197::araC PBADlacI TT; CmrThis study
    pYA4064pRE112 derived suicide vector for ΔrelA198::araC PBADlacI TT; CmrThis study
    pYA4092570-bp DNA encoding PsaA from aa 21 to aa 210 in pYA3627This study
    pYA4093570-bp DNA encoding PsaA from aa 21 to aa 210 in pYA3638This study
    pYA4359630-bp DNA encoding PsaA from aa 1 to aa 210 in pYA3342This study
    pYA4729927-bp DNA encoding PsaA from aa 1 to aa 309 in pYA3342This study
    pYA4730927-bp DNA encoding PsaA from aa 1 to aa 309 in pET-28aThis study
  • a Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance.