TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference
Strains
    N. gonorrhoeae strains
        FA19Wild-type; serum-resistant strain17
        FA19 lptA::spcSpectinomycin resistance insertion in lptA gene15
        FA19 potHI::KmrpotHI deletion with insertion of kanamycin cassetteThis study
        F62Clinical isolate; serum-sensitive strain27
    E. coli strains
        DH5αCloning strain; serum-sensitive strainInvitrogen
        MC4100Serum-sensitive strain; strain CGSC 6152Coli Genetic Strain Center
Plasmids
    pUC18KaphA-3 (kanamycin resistance) cassette in pUC1818
    pGEM-T EasyCloning vectorPromega