TABLE 5.

Infection of bovine dendritic cellsa by M. avium subsp. paratuberculosis

TimeNo. of intracellular bacteria% of inoculum
Uptake
    15 min(3 ± 0.4) × 1010.003
    30 min(6 ± 0.6) × 1020.01
Intracellular growth
    1 h(1.5 ± 0.3) × 104
    4 days(3.8 ± 0.3) × 1041.8
  • a A total of 1 × 106 monocyte-derived macrophages were incubated with human GM-CSF and IL-4 for maturation into dendritic cells.