TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotype (ORF) or descriptionReference and/or source
Strains
    E. coli
        BL21(DE3)FompT hsdSB(rB mB) gal dcm (DE3) 64; Invitrogen
        DH5αλ Φ80dlacZDM15 D(lacZYA-argF)U169 recA1 endA hsdR17(rK mK) supE44 thi-1 gyrA relA1Invitrogen
        XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac (F− proAB lacIqZΔM15 Tn10 (Tetr)Stratagene
    C. jejuni
        81-176Wild type; human isolate 4, 38; gift of Patricia Guerry
        CB1 flaA::aphA3 (cjj81176_1339::aphA3)Laboratory strain collection
        CB18 motA::aphA3 (cjj81176_0359::aphA3)This study
        CB21complemented aspA mutant; aspA::aphA3 (cj81176_1539::cat aspA)This study
        CB22complemented aspB mutant; aspB::aphA3 (cj81176_1539::cat aspB)This study
        CB25 peb1A::aphA3 (cjj81176_0928::aphA3)This study
        CB26 ciaB::aphA3 (cjj81176_0921::aphA3)This study
        CB30 sodB::aphA3 (cjj81176_0205::aphA3)This study
        CB43 cj0977::aphA3This study
        CB60 cjj81176_0996::aphA3This study
        CB61 cjj81176_0708::aphA3This study
        CB62 jlpA::aphA (cjj81176_1002::aphA3)This study
Plasmids
    pKETH-8cHis-tagged p480 (TnpA) transposase in pET expression vector; Kmr 59; gift of Nigel D. F. Grindley
    pTG426Source of Tn552cat; cat cassette flanked by 48-bp Tn552 terminal inverted repeats in pUC19 18; gift of Nigel D. F. Grindley
    pSB1699Source of Tn552kan-Campy; Tn552aphA3 in SpeI of pRY112 7
    pRY109Source of the chloramphenicol acetyltransferase gene (cat cassette) 74
    pBluescriptII SK(+/−)Phagemid cloning vector, f1 origin in plus orientation; lacPOZ′; Sac → Kpn polylinker orientation; AmprStratagene
    pSB3021 C. jejuni complementation vector; pGK2003 cjj81176_1539::cat 72
    pSB2996 cjj81176_1539::cat sodB in pSB3021This study
    pSB3000 motA::aphA3 in pBluescript II SK (+/−)This study
    pSB3001 peb1A::aphA3 in pBluescript II SK (+/−)This study
    pSB3002 cj0977::aphA3 in pBluescript II SK (+/−) (cj81176_0996::aphA3)This study