TABLE 3.

Translocation of M. avium subsp. paratuberculosis across an epithelial cell monolayer

Condition2 h6 h24 h
% of inoculum that translocatedTransmembrane resistance (Ω)a% of inoculum that translocatedTransmembrane resistance (Ω)% of inoculum that translocatedTransmembrane resistance (Ω)
No exposure to milk (5 × 105 bacteria)0.2 ± 0.05320 ± 100.4 ± 0.04c310 ± 300.6 ± 0.05330 ± 24
Preincubation with milk (2 × 105 bacteria)b0.4 ± 0.06320 ± 260.7 ± 0.04c,d328 ± 241.5 ± 0.06c,d325 ± 39
  • a Before the addition of bacteria, the transmembrane resistance was 320 Ω.

  • b M. avium subsp. paratuberculosis was incubated in the presence of whole milk for 2 h, washed, and used in the experiments.

  • c P < 0.05 for comparison with the previous time point.

  • d P < 0.05 for comparison with M. avium subsp. paratuberculosis incubated without milk.