TABLE 2.

ATCC mutants with survival defects in CSF

Functional group of proteinKnockoutGeneTranscript nameBroad database annotationaDescriptionMelaninCapsuleMutant phenotype(s)b
Transporter1A10 ENA1 ENA P-type ATPase 1CNAG_00531Cation ATPase transporter++Known to be
1F11c PXA2 Adrenoleukodystrophy proteinCNAG_02764ABC transporter++    hypovirulent
Transcription5C2 SRE1 Conserved hypothetical proteinCNAG_04804Helix-loop-helix DNA-binding domain; ergosterol biosynthesis++Known to be hypovirulent
Protein sorting/protein degradation10D3 VPS25 Conserved hypothetical proteinCNAG_04863ESCRT-II complex subunit; endosomal protein sorting++/−
7F4 RUB1 Ribosomal chaperoneCNAG_02827Ribosomal chaperone activity+Abnormal morphology;
5D3 CSN1201 Conserved hypothetical proteinCNAG_01697Transcription-associated recombination protein+/−+    slower growth
8F3 HRD1 SynoviolinCNAG_05469Transmembrane ubiquitin ligase required for endoplasmic reticulum-associated degradation; Zinc finger domain++
12F1 VAM6 RabGEFCNAG_05395Rab guanyl-nucleotide exchange factor activity+
Signaling4B10c NSR1 RNA splicing factor Pad-1CNAG_02130Binds nuclear localization sequences for pre-rRNA processing/biogenesis
13C5c CKB1 Casein kinase II beta chainCNAG_03590Regulation of cell cycle; cellular ion homeostasis; flocculation via cell wall protein-carbohydrate interaction; response to DNA damage stimulus+
14A4c PIK1 1-Phosphatidylinositol 4-kinaseCNAG_07744Inositol phosphate metabolism and phosphatidylinositol signaling system+++
14B2c SWE1 MYT1 kinaseCNAG_03171Protein kinase in regulation of cell cycle+++
Cell wall protein12G8 CPS1 Cellubiuronic acid synthaseCNAG_04320GlycotransferaseKnown to be hypovirulent
  • a The broad database uses the H99 background; ATCC mutants are in the CM018 background.

  • b Mutant phenotype initially characterized by Liu et al. (35).

  • c Novel host survival gene (not reported in initial screen) (35).