TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference
S. aureus
    RN4220Heavily mutagenized strain that accepts foreign DNA, agr mutant23
    SH10008325-4 with rsbU restored14
    COLA methicillin-resistant strain, isolated from a human infection in the early 1960s13
    NewmanMethicillin-sensitive laboratory strain, isolated from a human infection in 19523
    RN6390Laboratory strain related to strain 8325-425
    ALC4657SH1000 ΔrsrThis work
    ALC4658COL ΔrsrThis work
    ALC4659Newman ΔrsrThis work
    ALC4660RN6390 ΔrsrThis work
    ALC6582SH1000 Δrsr/rsr complementThis work
    ALC6583SH1000 ΔsarRThis work
    ALC6584SH1000 ΔsarR ΔrsrThis work
    ALC2386SH1000 agr::tetM14
    ALC6585SH1000 agr::tetM ΔrsrThis work
    ALC6586SH1000 with pEPSA5::rsrThis work
E. coli
    TOP10General cloning strainInvitrogen
Plasmids
    pMADE. coli-S. aureus shuttle vector containing a thermosensitive origin of replication bgaB, Emr, Apr2
    pEPSA5E. coli-S. aureus shuttle vector containing a xylose-inducible promoter, Apr, Cmr9
    pALC1484E. coli-S. aureus shuttle vector derived from pSK236 containing a multiple cloning site upstream of the gfpuvr gene (a gfp variant optimized for expression in S. aureus), Apr, Cmr16
    pALC1743agr P3 promoter driving the expression of gfpuvr in pALC148416
    pALC1741spa promoter driving the expression of gfpuvr in pALC148417
    pALC1740hla promoter driving the expression of gfpuvr in pALC148417
    pALC2831sspA promoter driving the expression of gfpuvr in pALC148426