TABLE 5.

Proteins present in ΔprsA2 prfA(L140F) mutant gels but absent from prfA(L140F) gels

Function or functional classGene no.Protein nameProtein descriptionNo. of peptide matches% coverageProtein scoreReference(s)
Virulence factorslmo1786InlCInternalin C82956819 and 24
lmo0204ActAActin assembly-inducing protein733181918 and 54
lmo0202LLOListeriolysin-O1244144483 and 86
lmo0205PlcBPhospholipase-C3173692 and 101
lmo0203MplZinc metalloproteinase precursor2917963, 64, 75, and 76
Cell surface and cell wall metabolismlmo2505P45Peptidoglycan lytic protein923437485
lmo2522LysM domain-containing protein53473674
lmo1547MreCRod shape-determining protein41146898
Transport/binding proteins and lipoproteinslmo2416ABC transporter, substrate binding protein211204
lmo0369Conserved hypothetical protein214109
lmo1388TcsACD4+ T cell-stimulating antigen (lipoprotein)26.922774
lmo1757Putative lipoprotein28.4395
lmo2637Hypothetical pheromone-like protein31368774
lmo1847ABC transporter, manganese binding protein415642
lmo1002PtsHPTS phosphocarrier protein (Hpr)44178214
Protein foldinglmo1474GrpEHeat shock protein/cochaperone436246
lmo1267TigTrigger factor (prolyl isomerase)82713797
Metabolism
    Carbohydrateslmo2455EnoEnolase1650374181
lmo2367PgiGlucose-6-phosphate isomerase26.2225
lmo2456PgmPhosphoglycerate mutase, 2,3-biphosphoglycerate independent10311668
lmo2459GapGlyceraldehyde 3-phosphate dehydrogenase28.641681
    Lipidslmo1808FabDMalonyl coenzyme A acyl carrier protein transacylase313296
    Coenzymeslmo2101Pyridoxine biosynthesis protein422744
    Amino acidslmo2414SufDHypothetical FeS assembly protein311246
    Nucleic acidslmo2611AdkAdenylate kinase526875
Detoxification/adaptation to atypical conditionslmo0927IspBSulfatase family protein613820110
lmo2785KatCatalase25.110226 and 58
lmo1439SodSuperoxide dismutase63727262 and 100
lmo2468ClpPATP-dependent Clp protease proteolytic subunit32060233 and 34
lmo0943FriNonheme iron-binding ferritin43618125
Regulation and transcriptionlmo0443Transcriptional regulator (putative)8281212
lmo1280CodYTranscriptional repressor73710504
lmo1496GreATranscription elongation factor544606
Protein synthesislmo0239CysSCysteinyl-tRNA synthetase27.628461
lmo1657TsfTranslation elongation factor Ts726853
lmo2654FusElongation factor G24.6479
lmo1314FrrRibosome recycling factor531676
lmo0250RplJ50S ribosomal protein L1042461727
lmo0251RplL50S ribosomal protein L7/L12220305
Amino acid synthesislmo0223CysKCysteine synthase A84363122
Specific pathwayslmo2556FbaAFructose bisphosphate aldolase5181045
lmo1339Glucokinase28.4363
lmo0429Glycosyl hydrolase28205
lmo0811Carbonic anhydrase211399