Table 1.

Strains used in the study

StrainLab IDRelevant propertiesSource
E. coli strains
    EPMax10B-pir116asd::GmrE1345Gmr; F λ mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK rpsL nupG Tn-pir116-FRT2 Δasd::Gmr-wFRTAvailable lab straina
    EPMax10B-pir116asdtrp::Gmr/ mob-KmrE1354Gmr Kmr; F λ mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK rpsL nupG Tn-pir116-FRT2 Δasd::wFRT Δtrp::Gmr-FRT5 mob[recA::RP4-2 Tc::Mu-Kmr]Available lab strain
    EPMax10B-lacIq/pirE1869F λ mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK rpsL nupG lacIq-FRT8 pir-FRT4Available lab strain
    EPMax10B-lacIq/pir/leu+E1889F λ mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 galU galK rpsL nupG lacIq-FRT8 pir-FRT4Available lab strain
B. pseudomallei strains
    1026b (wt)B0004Wild-type strain, clinical melioidosis isolate11
    1026b attTn7-gat-rfp (wt/rfp)B0015GSr; 1026b with mini-Tn7-gat-rfp inserted at glmS2 site29
    1026b-ΔasdBp::gat-FRTasd)B0011GSr; 1026b with gat-FRT cassette inserted in asdBp gene28
    1026b-ΔasdBp::gat-FRT/attTn7-bar-rfpasd/rfp)B0024GSr PPTr; 1026b ΔasdBp::gat-FRT mutant with mini-Tn7-bar-rfp inserted at glmS2 siteThis study
    1026b-ΔasdBp::gat-FRT/attTn7-bar-asdBp-rfpasd/complement/rfp)B0022GSr PPTr; 1026b ΔasdBp::gat-FRT mutant with mini-Tn7-bar-asdBp-rfp inserted at glmS2 siteThis study
  • a E. coli strains are available lab strains (when requesting, please use the Lab ID number).