Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescription and/or genotype or phenotypeaReference or source
Strain or plasmidDescription and/or genotype or phenotypeaReference or source
Bacterial strains
    P. aeruginosa
        PAO1Prototypical laboratory strain26
        relA mutantPAO1 with a gentamicin resistance cassette inserted into relA; Gmr20
        relA spoT mutantPAO1 relA mutant with an Ω streptomycin/spectinomycin resistance cassette inserted into spoT; Gmr Smr/SprThis study
    E. coli DH5αF φ80dlacZΔM15 Δ(lacZYAargF)U169 recA1 endA1 hsdR17(rK mK+) phoA supE44 λ thi-1 gyrA96 relA122
Plasmids
    pCR2.1TA cloning vector; Apr KmrInvitrogen
    pHP45ΩCloning vector pHP45 containing the Smr/Spr Ω element; Apr Smr/Spr43
    pEX18TcGene replacement vector containing the MCS from pUC18 and the sacB gene; Tcr25
    pSS11pEX18Tc containing a spoT::Ω insert; Tcr Smr/SprThis study
    pUCP28TE. coliP. aeruginosa shuttle vector derived from general cloning vector pUC18; Tpr47
    pSS6pUCP28T containing rpoZspoT from PAO1; TprThis study
  • a MCS, multiple cloning site.