Table 1.

Oligonucleotide primers used for mutant production

GeneOligonucleotide sequences of primersaEnzymeResistance cassette
argATCACTCGAGGCAAAGAGGTGTGCCGTGXhoIKm
GCCGCTGGGCCGCTGGGGTACCARGACGGCGTGG ATTKpnI
CTCGCCTCGTGCCATGGTACCCCAGCGGCKpnI
CGCAGATCTTAACCCTAAATCCGCCATCABglII
potGTCACTCGAGACGAAAGTGAAGAGCGGAXhoIKm
GATAAAAAGCTGGGTACCAGGATGCACCTTGAAKpnI
CGACCACCGAGGCATGGTACCCAGCTTTTTATCKpnI
CGGAGATCTCCGTCGGCACCACACAGCTCBglII
csgATCACTCGAGGGATCAAAACTATTGTCCGTXhoIKm
AATGCTCAGGTACCGCCGTTATGATTACCKpnI
ATAACGGCGGTACCTGAGCATTTATCAGTKpnI
CGCAGATCTTAGCGCAGACGCTAAATTAABglII
metFCGTCTCGAGGACATGAAGAAAATTCAACTXhoIKm
TTATTCCAGGTACCGCTCTTTGATGCCTTKpnI
CAAAGAGCGGTACCTGGAATAACGGTATCKpnI
TCCAGATCTTGGCAAATGGCATAACTCATBglII
ttrBTCACTCGACCGCTGATTCTCTGGAGGAXhoIKm
CTTGTACCGGGTACCCCGGCACAC AGGKpnI
GTCCCTGGATGCCTGGGTACCGGCCATAGGKpnI
CGCAGATCTTGGCAATGTGGACGGGAGBglII
ttrSTCACTCGACCCCGGCTTGTTGTTGATCXhoIKm
ACTGGGCCGGGTACCCGTCCACCAGTCKpnI
CCGCCTGAGCCGCATGGTACCCGCCCCAGTKpnI
GCGAGATCTTCATCCAGTAGATGAATBglII
pduATCACTCGACCCATGCGAGGTCTTTATGXhoIKm
CGCGGCGATGTTACCCGGTCAAAGKpnI
TGCATCGGTGGCCGCGGTAACATCGCCGCGKpnI
CGCAGATCTCCACCAGCTGACTGCTGCBglII
eutRTCACTCGACGAGAGCCTCCCCATCAATXhoIKm
GTGGCCAGCGTTACCTGCACAAAGCCCKpnI
CTAGCGCTGGAGGTAGTTACCGCTGGCGAGKpnI
CGGAGATCTGTCGGAGGGCCGGGCGTCBglII
btuBTCACTCGACAAGCCTGCGGCATCCTCCXhoIKm
CTCCGCTATGGTACCTTCCGATGCTATKpnI
GCGCTTTGTAGGAGGGGTACCATAGCGGAGKpnI
CGGAGATCTCGGTGGGACGAGGTTCAGBglII
cobSGATCTAGAACGAATCTGCTGTTTGCGCTXhoIKm
CAGCAGGGTACCTAGCGGAATACCACACCAGKpnI
CCGCTAGGTACCCTGCTGACCGGTGGTTTTCAKpnI
AGTCTAGAACAGAGCCAGCAGAAAGATCBglII
CAGCAGGGATCCTAGCGGAATACCACACCAGGBamH1Spc
CCGCTAGGATCCCTGCTGACCGGTGGTTTTCABamH1
cbiACATCTAGAAAGGCATCACGCATTTATTCXhoIKm
CGTTATGGTACCAATGGCATTTTTGAGGAGCTKpnI
GCCATTGGTACCATACGGTGATGTTAAAACATKpnI
TGTCTAGACAGCCAGTGCTGCACCATTTBglII
TAACATCACCGGATCCGCCGCCAGBamH1Spc
AGCAATCATGGCATGGGATCCGGTGATGTTBamH1
  • a Underlining and boldface indicate enzyme sites.