Table 1.

Preparation and characterization of S. enterica serovar Enteritidis COPS-conjugates used for immunization experiments in mice

Conjugate lot designationCarrier proteinPolysaccharide/protein conjugation ratio (wt/wt)aConjugation chemistryPurification schemeFinal polysaccharide/protein ratio (wt/wt)bMouse
O:H 1:1 lot 1Flagellin monomer1:1CDAPSEC0.78BALB/c and CD-1
O:H 1:1 lot 2Flagellin monomer1:1CDAPSEC-IEX0.26CD-1
O:H polymerFlagellar polymer1:1CDAPIEX0.56CD-1
O:H 10:1Flagellin monomer10:1CDAPSEC-IEX0.97CD-1
O:H Amox.Flagellin monomer3:1AminooxySEC-IEX0.45CD-1
O:CRM197CRM1971:1CDAPSEC-IEX0.55CD-1
  • a Ratio of polysaccharide to protein used in the conjugation reaction.

  • b Final ratio of polysaccharide to protein in the purified conjugate.