TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant propertiesSource or reference
Y. pestis strains
    KIM5Pgm Lcr+ pCD1 pMT1 pPCP1; conditionally virulent Δpgm 2.MED strain; also called KIM D27R. R. Brubaker
    KIM5-3003ΔlacZ derivative of KIM5; 1.2 kb of lacZ coding sequence deleted by allelic exchange with pLacZ3This study
    CO92.S6CO92 Apr Pgm+ Lcr+ pFra pPCP1; reconstituted virulent strain made by introduction of pCD2Ap into CO92.S112
    CO92.S19Reconstituted Lcr+ ΔyopM2 strain made by introducing pCD2Ap ΔyopM2 from CO99-3015.S11 into CO92.S166
E. coli strain
    DH5αλpirrecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1deoR Δ(lacZYA-argF)U169 λpir+; host strain for maintaining pLacZ3Lab stock
Plasmid
    pLacZ3Suicide allelic exchange plasmid for transferring internal 1.2-kb deletion into Y. pestis lacZ; 12.8 kb, R6K origin, SucB+ Smr ΔlacZ4