TABLE 1.

Strains and plasmids used in this research

Strain or plasmidRelevant characteristic(s)/genotypeSource or reference
Strains
    S. enterica serovar Typhimurium UK-1
        χ3761Wild-type UK-119
        χ8276ΔasdA1646
        χ8916ΔasdA16 ΔphoP23350
        χ9555ΔpabA1516 ΔpabB232 ΔasdA16 ΔaraBAD23 ΔrelA112391
        χ9241ΔpabA1516 ΔpabB232 ΔasdA16 ΔaraBAD23 ΔrelA198::araC PBAD lacI TT92
    S. pneumoniae WU2Wild-type virulent, encapsulated type 38
Plasmids
    pYA3342Asd+ vector, Ptrc, pBR ori46
    pYA3493Asd+ vector with β-lactamase N-terminal signal sequence, Ptrc, pBR ori46
    pYA4088Asd+ vector with β-lactamase N-terminal secretion signal specifying PspA Rx1 (amino acids 3-285) in pYA3493, Ptrc, pBR ori91
    pYA4569Asd+ vector with β-lactamase N-terminal secretion signal, PssaG, pBR oripYA3342
    pYA4570Asd+ vector with β-lactamase N-terminal secretion signal, PpagC, pBR oripYA3342
    pYA4571Asd+ vector with β-lactamase N-terminal secretion signal specifying PspA Rx1 (amino acids 3-285) in pYA4569, PssaG, pBR oripYA4569
    pYA4572Asd+ vector with β-lactamase N-terminal secretion signal specifying PspA Rx1 (amino acids 3-285) in pYA4570, PpagC, pBR oripYA4570