TABLE 2.

Oligonucleotides used in this study

OligonucleotideSequenceaSite(s)b
U741-F1gctctagaCCAGCCACGGCGCATACXbaI
U741-R1tcccccgggGACGGCATTTTGTTTACAGGSmaI
D741-F1tcccccgggCGCCAAGCAATAACCATTGSmaI
D741-R1cccgctcgagCAGCGTATCGAACCATGCXhoI
741-F2ggattccatatgGTGAATCGAACTGCCTTCNdeI
741-R2ccaatgcatTTATTGCTTGGCGGCAAGNsiI
EP1For1.1CGCggatcccatatgGTGAATCGAACTGCCTTCBamHI, NdeI
EP5RV1.4TGCATGCATTTACTGCTTGGCGGCAAGNsiI
EP2For1.4CGCggatcccatatgGTGAACCGAACTGCCTTCBamHI, NdeI
EP6RV2.1TGCATGCATCTACTGTTTGCCGGCGATNsiI
741v1fcgcggatcccatatgGTCGCCGCCGACATCGNdeI
741v1rcccgctcgagTTGCTTGGCGGCAAGGCXhoI
741v1.55fcgcggatcccatatgAGCAGCGGAGGCGGCGGNdeI
741v1.55rcccgctcgagCTGCTTGGCGGCAAGACCXhoI
741v2,3fcgcggatcccatatgGGCCCTGATTCTGACCGCCTGCAGCAGCGGAGGGTCGCCGCCGACATCGGNdeI
741v2rcccgctcgagCTGTTTGCCGGCGATGCCXhoI
741v3rgcccaagcttCTGTTTGCCGGCGATGCCHindIII
741v3.13fcgcggatcccatatgAGCAGCGGAAGCGGAAGCNdeI
741v3.13rcccgctcgagCTGTTTGCCGGCGATGCCXhoI
  • a Capital letters correspond to nucleotides of the meningococcal fHbp sequence (the gonococcus sequence moiety is reported in italics), and small letters correspond to nucleotides added for cloning reasons (underlined letters indicate sequences of restriction enzyme sites used for cloning PCR fragments).

  • b Enzymes for which the restriction sites are present in the sequence of the primer, added for cloning reasons.