TABLE 1

Bacterial strains and plasmids used in this studyd

Strain or plasmidDescriptionSource or reference(s)
Strains
    H10407Wild-type ETEC strain O78:H11; CFA/1; LT, STh, STp; EtpA28, 32, 64
    jf2656H10407 derivative with isogenic deletion of estPThis study
    jf2649H10407 derivative with isogenic deletion of estHThis study
    jf2847H10407 derivative with isogenic deletion of estP and estHThis study
    jf3038Deletion of astA on the large p948 virulence plasmid of H10407; KmrThis study
    jf3076jf2656 complemented with pQL230This study
    jf3081jf2649 complemented with pQL231This study
    JW5503-1E. coli K-12 in-frame ΔtolC732::Kmr58
    jf4652H010407 derivative with tolC::Kmr mutationThis study
    jf4709jf4652 with pBAD/mycHisBThis study
    jf4712jf4652 complemented with pYZ008This study
    jf4644jf2847 with pFLAG-CTC, Cmr Kmr AmprThis study
    jf4648jf2847 with pFLAG-STh, Cmr Kmr AmprThis study
    jf4651jf2847 with pSTh-FLAG, Cmr Kmr AmprThis study
    jf3626jf2847 with pQL239, Cmr Kmr AmprThis study
    AAEC191AAfimbriate E. coli K-12 derivative61
Plasmids
    pBAD/mycHisApBR322ori; PBAD; Ampr; araC; C-terminal myc epitope tag; arabinose-inducible expression plasmidInvitrogen
    pBAD/mycHisBpBR322ori; PBAD; Ampr; araC; C-terminal myc epitope tag; arabinose-inducible expression plasmidInvitrogen
    pKD13oriRSKγ Tn5 neomycin phosphotransferase (Kmr), FRT, β-lactamase (Ampr)56
    pKD46Lambda red recombinase helper plasmid56
    pQL239estP cloned into XhoI site of pBAD/myc-HisA in frame with myc-His tagsThis study
    pACYC184p15Aori, Cmr Tcr65
    pSMD001estH cloned into EcoRV site of pACYC184This study
    pGPS4oriRSKγ Cmr TcrNEB
    pETDuet-1pBR322ori; lacI; Ampr; IPTG-inducible expression plasmidNovagen
    pQL230estP cloned into BglII and XhoI sites of pETDeut-1This study
    pQL231estH cloned into NcoI and EcoRI sites of pETDeut-1This study
    pQL238estP cloned into BglII and XhoI sites and estH cloned into NcoI and EcoRI sites of pETDeut-1This study
    pGEX-4T1lacIq, Ampr; expression plasmid for N-terminal GST fusionsGE Healthcare Life Sciences
    pCW002a5,047-bp GST-STh expression plasmid, AmprThis study
    pCW003bestP cloned into BamHI and EcoRI sites of pGEXThis study
    pYZ011c5,047-bp GST-mSTh expression plasmid for mSTh (A14Q), AmprThis study, 25
    pYZ008H10407 tolC cloned into pBAD/mycHisBThis study
    pFLAG-CTC5.3-kb plasmid, expressing C-terminal FLAG fusion proteins, AmprSigma
    pFLAG3-STh5,609-bp Flag-pro-STh expression plasmid, AmprThis study
    pSTh-FLAG35,612-bp pro-STh-Flag expression plasmid, AmprThis study