TABLE 1

Strains and plasmids used in this study

Strain or plasmidCharacteristic(s)aReference or source
Strains
    S. aureus
        TCH1516Community-associated USA300 MRSA isolate44
        RN4220Restriction-deficient transformation recipient45
        RKC0323TCH1516 ΔppiBThis work
        RKC0505TCH1516 PpiB F64AThis work
        RKC0165TCH1516 agrB17
        RKC0183TCH1516 hlaThis work
        NE1354USA300 JE2 hla::Bursa; hla NARSA transposon mutant41
    E. coli
        DH5αCloning strainInvitrogen
        BL21(DE3)/pLysSProtein expression strainPromega
        NEB ExpressProtein expression strainNEB
Plasmids
    pMK4Gram-positive shuttle vector (Cmr)46
    pJB38Temperature-sensitive allelic exchange plasmid (Cmr)19
    pET24dC-terminal His6 tag expression vectorNovagen
    pMALc5XN-terminal MBP fusion expression vectorNEB
    pRKC0276pET24d nuc8
    pRKC0212pMALc5X ppiB8
    pRKC0376pMALc5X ppiB H58AThis work
    pRKC0377pMALc5X ppiB R59AThis work
    pRKC0378pMALc5X ppiB F64AThis work
    pRKC0379pMALc5X ppiB Q114AThis work
    pRKC0380pMALc5X ppiB F116AThis work
    pRKC0381pMALc5X ppiB W152AThis work
    pRKC0295pJB38 containing DNA flanking ppiB geneThis work
    pRKC0438pJB38 containing ppiB F64A alleleThis work
  • a MRSA, methicillin-resistant S. aureus; Cmr, chloramphenicol resistant.