TABLE 1.

Bacterial strains and plasmids

Strain or plasmidCharacteristicsSource or reference
E. coli strains
    DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdr17(rK mK+) deoR thi-1 supE44 gyrA96 relA1Life Technologies
    One-Shot TOP10F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(Strr) endA1 λInvitrogen
    HB101thi-1 hsdS20(rB mB) supE44 recAB ara-14 leuB5 proA2 lacY1 galK rpsL20 (Smr) xyl-5 mtl-18
C. sordellii strains
    ATCC 9714Type strain; TcsL+ TcsH Neu+ SDL PLC+23
    DA108Clinical isolate; TcsL negative24 30
    DLL5001ATCC 9714 tcsL Ω targetron (mutant 1); erythromycin resistantThis study
    DLL5002ATCC 9714 tcsL Ω targetron (mutant 2); erythromycin resistantThis study
    DLL5003ATCC 9714 tcsL Ω targetron (mutant 3); erythromycin resistantThis study
Plasmids
    pMTL9361CmE. coli-C. difficile mobilizable shuttle vector; carries pCD6 replication region, oriT, and Tmr10
    pVS520Tra+ Mob+ RP4 derivative; Tcr45
    pJIR3566Clostridial mobilizable targetron vector; carries pCB102 replication region, the lacZα gene, oriT, and Cmr13
    pDLL2Group II intron of pJIR3566 retargeted to the 2993/2994s site of the tcsL geneThis study