TABLE 1

Genes with increased expression in WT A. baumannii following treatment with H2O2

Category and locusAnnotation (KEGG)aFold change
Peroxide detoxification
    A1S_1459Alkyl hydroperoxide reductase subunit F (ahpF2)61.9
    A1S_1200Alkyl hydroperoxide reductase subunit F (ahpF1)49.3
    A1S_1458Alkyl hydroperoxide reductase subunit F (ahpF2)47.2
    A1S_1201Alkyl hydroperoxide reductase subunit F (ahpF1)40.3
    A1S_1460Alkyl hydroperoxide reductase subunit F (ahpF2)35.7
    A1S_3382Catalase (katE)27.7
    A1S_2863Putative antioxidant protein5.9
Sulfur homeostasis
    A1S_2533Putative esterase459.7
    A1S_2532Sulfate transport protein364.5
    A1S_2531Sulfate transport system substrate-binding protein334.4
    A1S_2534Sulfate transport system permease protein172.9
    A1S_0030Sulfonate transport system substrate-binding protein165.8
    A1S_0028Alkanesulfonate monooxygenase113.7
    A1S_2535Sulfate transport system permease protein106.1
    A1S_0029Sulfonate transport system substrate-binding protein71.2
    A1S_0027Sulfonate transport system permease protein49.6
    A1S_3207Sulfate transport system substrate-binding protein46.0
    A1S_1408Putative rhodanese-related sulfurtransferase25.5
    A1S_2536Sulfate transport system ATP-binding protein18.1
    A1S_0977Arylsulfatase18.0
    A1S_3306Dimethylsulfone monooxygenase17.9
    A1S_0026Sulfonate transport system ATP-binding protein16.2
    A1S_2846Sulfite reductase (NADPH) hemoprotein beta-component5.6
Iron homeostasis
    A1S_2389Iron complex transport system permease protein26.2
    A1S_23812,3-Dihydroxybenzoate-AMP ligase21.8
    A1S_17194Fe-4S ferredoxin iron-sulfur binding21.4
    A1S_2380Bifunctional isochorismate lyase18.2
    A1S_2379Histidine decarboxylase16.3
    A1S_2382BasD16.0
    A1S_2388Iron complex transport system permease protein15.8
    A1S_2387Iron complex transport system ATP-binding protein14.9
    A1S_2390Putative acinetobactin biosynthesis protein8.7
    A1S_2372Isochorismate synthase8.2
    A1S_2386Iron complex transport system substrate-binding protein7.4
    A1S_2383Putative acinetobactin biosynthesis protein6.9
    A1S_2392Putative acinetobactin utilization protein6.1
    A1S_2077Putative outer membrane porin receptor for Fe(III)-coprogen, Fe(III)-ferrioxamine B, and Fe(III)-rhodotorulic acid uptake (fhuE)5.7
    A1S_3339Iron complex outer membrane receptor protein5.4
    A1S_1607Iron complex outer membrane receptor protein4.6
    A1S_1647Putative siderophore biosynthesis protein4.4
    A1S_1359Iron(III) transport system substrate-binding protein4.3
    A1S_25304-Amino-4-deoxychorismate lyase4.1
    A1S_1634Rrf2 family transcriptional regulator4.1
Phosphate transport
    A1S_2448Phosphate transport system substrate-binding protein10.5
    A1S_2447Phosphate transport system permease protein6.8
    A1S_3374Positive Pho regulon response regulator6.0
    A1S_3376Phosphate regulon sensor histidine kinase PhoR4.7
    A1S_0256Phosphate transport system protein4.4
Nucleic acid synthesis and repair
    A1S_2273RNA 3′-terminal phosphate cyclase (ATP)26.4
    A1S_2586dGTP triphosphohydrolase8.6
    A1S_0310Excinuclease ABC subunit C6.4
    A1S_1173DNA polymerase V5.3
    A1S_2008DNA polymerase V4.8
    A1S_0636DNA polymerase V4.8
    A1S_1174DNA polymerase V4.6
    A1S_1962Recombination protein RecA4.4
    A1S_3295Excinuclease ABC subunit A4.2
    A1S_3359Topoisomerase IV subunit B4.1
Amino acid transport and metabolism
    A1S_1443Taurine transport system ATP-binding protein222.8
    A1S_0023Putative malic acid transport protein135.0
    A1S_1397Polar amino acid transport system permease protein102.2
    A1S_1444Taurine transport system permease protein87.3
    A1S_1442Taurine transport system substrate-binding protein85.9
    A1S_1396Polar amino acid transport system permease protein76.5
    A1S_1445Taurine dioxygenase41.8
    A1S_1398Polar amino acid transport system ATP-binding protein27.1
    A1S_0921Arginine:ornithine antiporter/lysine permease14.7
    A1S_1399Polar amino acid transport system substrate-binding protein11.4
    A1S_1485d-Methionine transport system substrate-binding protein9.9
    A1S_2384Lysine N6-hydroxylase6.6
    A1S_1046Lysine exporter6.3
    A1S_1407Serine O-acetyltransferase6.1
    A1S_1400Polar amino acid transport system substrate-binding protein4.8
Ribosome
    A1S_2271tRNA-splicing ligase RtcB118.6
    A1S_1961Ribosome-associated heat shock protein Hsp155.1
Fatty acid metabolism
    A1S_1436Putative acyl-CoA dehydrogenase7.8
    A1S_1437Putative acyl-CoA dehydrogenase7.7
    A1S_2458Linoleoyl-CoA desaturase7.5
    A1S_0394Putative acyl-CoA dehydrogenase7.4
Transport
    A1S_1456Chromate transporter23.1
    A1S_1457Chromate transporter13.1
    A1S_2378Putative ABC transporter8.4
    A1S_1720NitT/TauT family transport system substrate-binding protein8.2
    A1S_3272MFS transporter, YNFM family, putative membrane transport protein5.7
    A1S_2377Putative ABC transporter5.4
    A1S_2311ABC-2 type transport system ATP-binding protein4.4
    A1S_1772MFS transporter, DHA2 family, multidrug resistance protein4.2
Metabolism (other)
    A1S_1488Putative acyl-CoA dehydrogenase100.9
    A1S_1487Putative acyl-CoA dehydrogenase30.0
    A1S_3305FMN reductase24.6
    A1S_1486Putative monooxygenase (DszA-like)24.5
    A1S_0922Putative homocysteine S-methyltransferase family protein19.9
    A1S_0393Putative acyl-CoA dehydrogenase15.3
    A1S_0024Adenosylhomocysteine nucleosidase10.9
    A1S_0463Putative alkaline phosphatase9.0
    A1S_2459Putative oxidoreductase8.3
    A1S_3222Homocysteine synthase6.9
    A1S_2293Ferredoxin/flavodoxin–NADP+ reductase5.5
    A1S_1446Allantoin racemase4.4
    A1S_0131Streptomycin 3"-adenylyltransferase4.3
Other
    A1S_1717GntR family transcriptional regulator32.1
    A1S_1414LrgB-like protein27.5
    A1S_0466Sec-independent protein translocase protein TatA25.0
    A1S_1665Putative membrane protein19.9
    A1S_3114CBS domain-containing membrane protein19.0
    A1S_1677Putative porin precursor17.5
    A1S_3381AnkB protein17.4
    A1S_0465Sec-independent protein translocase protein TatB16.1
    A1S_1963Regulatory protein13.2
    A1S_0464Sec-independent translocation protein TatC12.8
    A1S_1928Putative signal peptide12.4
    A1S_2473Transcriptional regulator, LysR family10.4
    A1S_1224Transposase8.9
    A1S_0416Putative transcriptional regulator (LysR family)5.9
    A1S_3326Putative membrane protein5.4
    A1S_1438Putative coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase5.2
    A1S_3375Phosphate regulon response regulator PhoB5.2
    A1S_1539ArsR family transcriptional regulator4.7
    A1S_1767Putative acid phosphatase4.7
    A1S_1503Transmembrane pair4.5
    A1S_3360Putative esterase4.3
    A1S_2319Putative membrane protein4.2
    A1S_0565Putative membrane protein4.2
    A1S_1197Putative extracellular nuclease4.1
Hypothetical
    A1S_0517Hypothetical protein139.3
    A1S_3813Hypothetical protein73.1
    A1S_3666Hypothetical protein47.7
    A1S_3906Hypothetical protein43.9
    A1S_1718Hypothetical protein30.1
    A1S_2272Hypothetical protein26.2
    A1S_3783Hypothetical protein21.5
    A1S_3116Hypothetical protein15.4
    A1S_3115Hypothetical protein14.6
    A1S_3668Hypothetical protein13.6
    A1S_3804Hypothetical protein12.5
    A1S_0617Hypothetical protein12.2
    A1S_3782Hypothetical protein9.5
    A1S_3913Hypothetical protein8.1
    A1S_0889Hypothetical protein8.0
    A1S_1390Hypothetical protein7.7
    A1S_0976Hypothetical protein7.0
    A1S_2266Hypothetical protein6.9
    A1S_3113Hypothetical protein5.9
    A1S_0224Hypothetical protein5.6
    A1S_1614Hypothetical protein5.5
    A1S_3878Hypothetical protein5.5
    A1S_3479Hypothetical protein5.2
    A1S_3780Hypothetical protein5.1
    A1S_3682Hypothetical protein5.1
    A1S_2267Hypothetical protein4.9
    A1S_3912Hypothetical protein4.9
    A1S_3638Hypothetical protein4.7
    A1S_3849Hypothetical protein4.1
    A1S_0770Hypothetical protein4.1
    A1S_3513Hypothetical protein4.1
  • a CoA, coenzyme A; MFS, major facilitator superfamily; CBS, cystathionine β-synthase; FMN, flavin mononucleotide.