TABLE 1

Strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
 Escherichia coli DH5αΔ(lacZYA-argF)U169 φ80dlacZΔM15Lab collection
 Edwardsiella piscicida
        EIB202Edwardsiella piscicida wild-type strain, Colr CmrCCTCC no. M208068
        ΔeseHEIB202 with deletion of eseH8
        ΔeseH::pEseHThe ΔeseH strain complemented with pUTt-0456-EseH-HA, Ampr8
Plasmids
    pCDH-HApCDH-CMV-MCS derivative containing HA tag8
    pCDH-EseH-HApCDH-CMV-MCS derivative containing EseH-HA fusion8
    pCDH-EseH (K79R)-HApCDH-CMV-MCS derivative containing EseH(K79R)-HA fusion8
    pCDH-EseH (H81R)-HApCDH-CMV-MCS derivative containing EseH(H81R)-HA fusion8
    pCDH-EseH (K111A)-HApCDH-CMV-MCS derivative containing EseH(K111A)-HA fusion8
    pCDH-OspF-HApCDH-CMV-MCS derivative containing OspF-HA fusionThis study
    pCDH-OspF (K102R)-HApCDH-CMV-MCS derivative containing OspF (K102R)-HA fusionThis study
    pCDH-OspF (H104R)-HApCDH-CMV-MCS derivative containing OspF (H104R)-HA fusionThis study
    pCDH-OspF(K134A)-HApCDH-CMV-MCS derivative containing OspF(K134A)-HA fusionThis study
    pCDH-EGFPpCDH-CMV-MCS derivative containing EGFP tagThis study
    pCDH-EseH-EGFPpCDH-CMV-MCS derivative containing EseH-EGFP fusionThis study
    pCDH-EseH (K79R)-EGFPpCDH-CMV-MCS derivative containing EseH(K79R)-EGFP fusionThis study
    pCDH-EseH (H81R)-EGFPpCDH-CMV-MCS derivative containing EseH(H81R)-EGFP fusionThis study
    pCDH-EseH (K111A)-EGFPpCDH-CMV-MCS derivative containing EseH(K111A)-EGFP fusionThis study
    pCDH-OspF-EGFPpCDH-CMV-MCS derivative containing OspF-EGFP fusionThis study
    pCDH-OspF (K102R)-EGFPpCDH-CMV-MCS derivative containing OspF (K102R)-EGFP fusionThis study
    pCDH-OspF (H104R)-EGFPpCDH-CMV-MCS derivative containing OspF (H104R)-EGFP fusionThis study
    pCDH-OspF (K134A)-EGFPpCDH-CMV-MCS derivative containing OspF (K134A)-EGFP fusionThis study
    pCDH-p65-FlagpCDH-CMV-MCS derivative containing p65-Flag fusionThis study
    pCDH-p65 (S276A)-FlagpCDH-CMV-MCS derivative containing p65 (S276A)-Flag fusionThis study
    pRK5-HA-ubiquitin-48Mammalian expression of HA-tagged ubiquitin with only K48, AmprAddgene