TABLE 4

Bacterial strains used in this study

StrainRelevant characteristicsReference or source
Acinetobacter baumannii 17978Wild-type Acinetobacter baumanniiATCC
Acinetobacter baumannii 17978 ΔoxyRIn-frame ΔoxyR::aphAThis study
Acinetobacter baumannii 17978 pWH1266Empty-vector control with plasmid pPr01WH126678
Acinetobacter baumannii 17978 ΔoxyR pWH1266Empty-vector control with plasmid pPr01WH1266This study
Acinetobacter baumannii 17978 ΔoxyR poxyRWH1266Complementation strain containing plasmid p.Pr01.oxyR.WH1266This study
Acinetobacter baumannii 17978 ΔoxyR poxyR C202S WH1266Complementation strain containing plasmid p.Pr01.oxyR.WH1266 with C202S mutationThis study
Acinetobacter baumannii 17978 pAT02Wild type harboring recAb machinery for recombineering33
Escherichia coli DH5α p.oxyRCloning strain containing plasmid p.Pr01.oxyR.WH1266This study
Escherichia coli DH5α p.oxyR C202SCloning strain containing plasmid p.Pr01.oxyR.WH1266 with C202S mutationThis study
Escherichia coli DH5α p.oxyR pCRBluntCloning strain containing oxyR in pCR-BluntThis study
Escherichia coli DH5α p.oxyR C202S pCRBluntCloning strain containing oxyR with C202S mutation in pCR-BluntThis study
Escherichia coli BL21(DE3) p.oxyR.ET15BProtein expressing strain for oxyR cloned into pET15bThis study
Escherichia coli DH5α p.oxyR.ET15BCloning strain for oxyR cloned into pET15bThis study
Escherichia coli BL21(DE3) p.oxyRC202S.ET15BProtein-expressing strain for OxyR C202S cloned into pET15bThis study
Escherichia coli DH5α p.oxyRC202S.ET15BCloning strain for oxyR C202S cloned into pET15bThis study
Acinetobacter baumannii AB5075Wild-type parental strain for transposon library38
Acinetobacter baumannii AB07598AB5075 transposon mutant ABUW_2905-184::T2638
Acinetobacter baumannii AB07599AB5075 transposon mutant ABUW_2905-143::T2638
Acinetobacter baumannii AB07600AB5075 transposon mutant ABUW_2905-146::T2638
Acinetobacter baumannii AB07601AB5075 transposon mutant ABUW_2905-156::T2638