TABLE 1

Wild-type strains and mutant L. pneumophila strains used in this study

Strain or plasmidDescriptionSource or reference
Strains
 L. pneumophila CorbyWild-type strain61
    L. pneumophila Corby ΔdotAdotA::Tn5 mutant of L. pneumophila CorbyKindly provided by Antje Flieger,
Robert-Koch-Institut, Wernigerode, Germany
    L. pneumophila Corby Δmipmip::nptI mutant of L. pneumophila Corby40
    L. pneumophila Corby ΔppiBppiB::nptI mutant of L. pneumophila CorbyThis study
    L. pneumophila Corby Δmip ΔppiB (ΔΔ)mip::nptI and ppiB::cat mutant of L. pneumophila CorbyThis study
    L. pneumophila Corby ΔppiAppiA::aac1 mutant of L. pneumophila CorbyThis study
    L. pneumophila Corby ΔppiA ΔppiBppiA::aac1 and ppiB::nptI mutant of L. pneumophila CorbyThis study
Complemented strains
    WT(+)Wild-type strain carrying empty pBCKS plasmidThis study
    Mip(+)mip::nptI mutant of L. pneumophila Corby carrying pEWM10340
    PpiB(+)ppiB::nptI mutant of L. pneumophila Corby carrying pJRA1 ppiB
cloned in pBCKS with SacI and KpnI
This study
pBCKS complementation vectorpUC ori Cmr (cat)Stratagene (62)