TABLE 5.

Time course showing the impacts of inhibitor B8I-2 at 10 μM on intracellular replication in macrophages and on cell survival

Time (h)Presence of B8I-2 (10 μM)bAvg replicationaSD% Cytotoxicity
14.600.110
1+4.540.110
54.380.090
5+4.630.150
243.560.190
24+1.240.690
485.310.200
48+1.911.780
  • a Intracellular growth of B. abortus 2308 was quantified by lysis of macrophages, dilution, and plating (replication is shown on a logarithmic scale). The toxicity of B8I-2 was assessed using the cytotox96 kit (Promega). Averages from two replicates with three wells per replicate are shown.

  • b +, present; −, absent.