TABLE 1

H. pylori strains and plasmids used in this study

Plasmid or strainDescriptionSource or reference
Plasmids
    pMCHBHpUC57 containing mCherryThis study
    pcagA-MCHpGEM T Easy vector containing cagA 3′ end::mCherry transcriptional fusionThis study
    pcagA-MCHCATpcagA-MCH containing catThis study
    p5056cagA-3pGEM T Easy vector containing cagA, contains sequences 500 bp upstream and 500 bp downstream of the cagA ATG initiation codon of PZ5056This study
    pcagA::cat-rpsLp5056cagA-3, contains cagA::cat rpsLThis study
    p5056cagAmut1p5056cagA-3 with a mutation in the +59 motif and stem B, resulting in an altered stem B structure (Fig. S4)This study
    p5056cagAmut4p5056cagA-3 with a mutation in the +59 motif and stem B, resulting in an altered stem B structure (Fig. S4)This study
    p5056cagAmutstem1p5056cagA-3 with a mutation in stem B of the cagA 5′ UTR; the structure of stem B is retained (Fig. S4)This study
    p5056cagAmutstem2p5056cagA-3 with a mutation in stem-loop B of the cagA 5′ UTR; the structure of stem B is retained (Fig. S4)This study
    p5056cagAmutstem3p5056cagA-3 with a mutation in stem B of the cagA 5′ UTR, resulting in an altered stem B structure (Fig. S4)This study
    p5056cagAmutloopp5056cagA-3 with a mutation in the loop of stem-loop B (Fig. S4)This study
    p5056cagAmutmotif1p5056cagA-3 with a mutation in the +59 motif (Fig. S4)This study
    p5056cagAmutΔmotifp5056cagA-3 with a deletion of the +59 motif (Fig. S4)This study
H. pylori strains
    26695Wild-type H. pylori48
    26695-cagApromΔ(−66 to −26)26695 containing deletion of cagA promoter, ΔrdxA27
    26695-cagApromΔ(−214 to −26)26695 containing deletion of cagA promoter, ΔrdxA27
    26695-cagA5056WT26695 containing the 1-kb promoter fragment of cagA from H. pylori PZ5056, ΔrdxA23
    26695-cagA5056mut126695 containing the 1-kb promoter fragment of cagA from H. pylori PZ5056, mut1 mutation, ΔrdxA23
    HpcagAMCH126695 containing cagA-mCherry-catThis study
    HpcagAMCH226695-cagA5056WT containing cagA-mCherry-catThis study
    HpcagAMCH326695-cagA5056mut1 containing cagA-mCherry-catThis study
    HpcagAMCH426695-cagApromΔ(−66 to −26) containing cagA-mCherry-catThis study
    HpcagAMCH526695-cagApromΔ(−214 to −26) containing cagA-mCherry-catThis study
    PZ5056Clinical isolate from a gastric cancer patient23, 30
    PZ5056 rpsL-K43RPZ5056 rpsl-K43RThis study
    PZ5056-cagAcat-rpsLPZ5056 rpsL-K43R containing cagA::cat-rpsLThis study
    PZ5056-cagARPZ5056 rpsL-K43R with a restored +59 motifThis study
    PZ5056-cagAmut1PZ5056 rpsL-K43R with the cagAmut1 mutationThis study
    PZ5056-cagAmut4PZ5056 rpsL-K43R with the cagAmut4 mutationThis study
    PZ5056-cagAmutstem1PZ5056 rpsL-K43R with the cagAmutstem1 mutationThis study
    PZ5056-cagAmutstem2PZ5056 rpsL-K43R with the cagAmutstem2 mutationThis study
    PZ5056-cagAmutloopPZ5056 rpsL-K43R with the cagAmutloop mutationThis study
    PZ5056-cagAmutmotif1PZ5056 rpsL-K43R with the cagAmutmotif1 mutationThis study
    PZ5056-cagAmutΔmotifPZ5056 rpsL-K43R with deletion of the +59 motifThis study