TABLE 1

Physicochemical properties of CS6 subunit fusions

ProteinTheoretical mol wta (kDa)Purityb (%)Yieldc (mg/g)Tm (°C)HPLC-SEC
Peak retention time(s) (min)Mol wt (kDa)dOligomeric state
CssA fusions
    dscACssA18.5951.35510.420.9Monomeric
    dscACssAA34.0951.349.9, 75.39.334.2Monomeric
    dscBCssAB35.0988.958.8, 74.57.5, 8.0, 9.245.7, 94.6Multimeric
    dscACssABA50.4952.154.3, 758.559.2Monomeric
    dscBCssABB51.3944.059.5, 747.2, 7.5, 8.567.4, 132Multimeric
Refined CssAB fusions
    dscACssAB34.8974.9709.235.5Monomeric
    ntd_dscBCssAB33.3993.859, 74.69.334.1Monomeric
    ntd_dscACssAB33.2973.769.7, 76.19.433.9Monomeric
CssB fusions
    dscBCssB19.4913.160.110.422.5Monomeric
    dscBCssBB35.81002.758, 92.68.1, 9.237.5, 94Multimeric
    dscACssBA34.8914.369.2, 74.77.7, 8.2, 9.539.8, 93Multimeric
    dscACssBAA50.4901.071.8, 77.67.1, 8.0141Multimeric
    dscBCssBAB51.3891.259.7, 75.57.6, 8.0, 8.663.1Multimeric
    dscACssBBA51.2910.559, 71.4, 767.2, 7.9, 8.965.4, 122.7Multimeric
Refined CssBA fusions
    dscBCssBA35.0978.274.19.437.3Monomeric
    ntd_dscACssBA33.2970.576.19.433.7Monomeric
    ntd_dscBCssBA33.3983.074.79.333.9Monomeric
  • a The theoretical molecular weight of each protein was determined from the primary amino acid sequence.

  • b Protein purity was determined by densitometric measurements of the protein separated on an SDS-PAGE gel.

  • c Milligrams of protein obtained per gram of wet bacterial cell paste lysed.

  • d The experimental molecular weight was determined using the refractive index of molecules eluting from the HPLC-SEC column.