TABLE 1

Primers used in this study

GeneRepeat unitNo. of repeats indicating ON or OFFPrimer sequenceaReference
lgtCGACA10 = ON; 11 and 12 = OFFFor: 5′-VIC-TCATCGAGCAAAGGCATTG-3′19
Rev: 5′-CTTACAGCTAAATAAGGTGC-3′
lex2AGCAA10 and 11 = OFF; 12 = ONFor: 5′-NED-CGGAATTATGTTAATCAC-3′19
Rev: 5′-GTTTGCTTTGTGATGTAC-3′
lic2ACAAT10 = ON; 11 and 12 = OFFFor: 5′-FAM-ACTGAACGTCGCAAA-3′24
Rev: 5′-GCTAATTAAACAGCCT-3′
lic1ACAAT10 and 11 = OFF; 12 = ONFor: 5′-VIC-CAAAAATAACTTTAACGTG-3′19
Rev: 5′-AATGCTGATGAAGAAAATG-3′
lic3ACAAT10 and 11 = OFF; 12 = ONFor: 5′-NED-ATTACCTGCAATAATGACAG-3′21
Rev: 5′-TATTCAATGAACGGTAGAAT-3′
Lic3A specific: 5′-GCCAGTAGTCGCAAAAGTGTC-3′
lic3BCAAT11 = ON; 12 and 13 = OFFFor: 5′-NED-ATTACCTGCAATAATGACAG-3′21
Rev: 5′-TATTCAATGAACGGTAGAAT-3′
Lic3B specific: 5′-TCAAACATCTTGCCGTCTTC-3′
oafAGCAA9 and 10 = OFF; 11 = ONFor: 5′-FAM-GCCTAATATTTATTATCTCTC-3′28
Rev: 5′-GTATGAATAATTAATGCTG-3′
modAAGCC or AGTC10 = ON; 11 and 12 = OFFFor: 5′-FAM-ATGGCGGGCAAAGCACCGAAGA-3′46
Rev: 5′-CAAAAAGCCGGTCAATTTCATCAAA-3′
  • a For, forward; Rev, reverse.