TABLE 1.

Anaplasma marginale St. Maries strain proteins bound by serum IgG2 induced by live vaccination

Protein typeSpot no.aProtein annotationMascot score(s) (standard)bNo. of peptides with P values of <0.05% sequence coverageMol wt (kDa)Theoretical pI
Membrane727AM1220 Omp7843941.98.9
727cAM1221 Omp8421242.99.2
122, 727cAM1222 Omp979, 422, 16, 243.18.2
424, 425, 426AM1255 Omp11534, 267, 36510, 5, 935, 23, 3334.86.7
421, 715AM1258 Omp13139, 653, 16, 1238.07.6
727AM075 Omp14301243.17.6
710, 411, 712AM779 surface-expressed protein128, 38, 653, 1, 18, 2, 357.47.2
724AM854 peptidoglycan-associated lipoprotein13831425.95.3
123-130AM1144 Msp2132-6402-167-1642.16.0
30, 112, 113AM1063 Msp3593, 84, 4814, 2, 114, 2, 2104.56.0
Housekeeping122AM231 succinyl-CoA synthetase beta chain (SucC)9211141.85.5
121, 122AM254 elongation factor Tu (TufB)647, 42214, 836, 3043.05.4
702AM1131 isocitrate dehydrogenase (Icd)4011153.55.8
116, 117AM944 Hsp chaperonin (GroEL)480, 2189, 525, 1858.45.2
701, 703, 411AM1174 F1-ATP synthase alpha subunit (AtpA)290, 188, 297, 3, 121, 7, 154.66.0
  • a Spot no. refers to the identification illustrated in Fig. 1.

  • b Standard MASCOT scores are the sum of probability-based MASCOT ion scores for each individual peptide.

  • c OMP8 and OMP9 hits for spot no. 727 are from the same peptide.